April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Application of the TLR9 Ligand CpG DNA to the Injured Corneal Epithelium Induces Intraocular Inflammation
Author Affiliations & Notes
  • H. R. Chinnery
    Centre Ophthalmology and Visual Sciences, Lions Eye Institute, Nedlands, Australia
  • S. McLenachan
    Centre Ophthalmology and Visual Sciences, Lions Eye Institute, Nedlands, Australia
  • M. Degli-Esposti
    Centre Ophthalmology and Visual Sciences, Lions Eye Institute, Nedlands, Australia
  • J. V. Forrester
    Div of Applied Medicine, Imm & Inf, University of Aberdeen, Aberdeen, United Kingdom
  • E. Pearlman
    Ophthalmology and Visual Sciences, Case Western Reserve University, Cleveland, Ohio
  • P. G. McMenamin
    Anatomy & Human Biology/Ct Ophth Vis Sci, Univ of Western Australia, Perth, Australia
  • Footnotes
    Commercial Relationships  H.R. Chinnery, None; S. McLenachan, None; M. Degli-Esposti, None; J.V. Forrester, None; E. Pearlman, None; P.G. McMenamin, None.
  • Footnotes
    Support  NH&MRC Grant 572709
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 2019. doi:
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      H. R. Chinnery, S. McLenachan, M. Degli-Esposti, J. V. Forrester, E. Pearlman, P. G. McMenamin; Application of the TLR9 Ligand CpG DNA to the Injured Corneal Epithelium Induces Intraocular Inflammation. Invest. Ophthalmol. Vis. Sci. 2010;51(13):2019.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : CpG oligodeoxynucleotides (ODN) are synthetic immunostimulatory sequences containing an abundance of CpG-DNA motifs similar to those found in high quantities in bacteria and viruses. Recognition of CpG DNA by TLR9 leads to activation of the innate immune system. We investigated the inflammatory response to CpG DNA in the mouse eye following its application to the injured corneal epithelium.

Methods: : The central corneal epithelium of C57Bl/6 mice (aged 6-12 weeks) was debrided using an Algerbrush. 20ug of CpG ODN 1826 (Type ‘B’), or control (GpC) ODN was applied to the cornea. Mice were sacrificed at 0h, 0.5h, 2h, 6h, 24h, 72h or 7 days after treatment. Frozen eye sections or corneal, retinal and iris/ciliary body wholemounts were examined for infiltration of neutrophils and macrophages following immunostaining with anti-leukocyte antibodies (NIMP, Iba-1, MHC Class II and F4/80). Macrophages and neutrophils were quantified in the cornea and retina/vitreous.

Results: : In the cornea, neutrophils had infiltrated by 6h, reaching a peak at 24h and were absent by 72h. At 24h, F4/80+ macrophages were present in the corneal stroma, but their density increased over 6-fold by Day 7. A large influx of neutrophils and macrophages were present in the anterior chamber, ciliary body, iris, vitreous and inner retina. Quantitation of neutrophils in the retina/vitreous in wholemounts revealed a small number of intravascular neutrophils by 6h (average 15 cells/mm2), increasing to 800 cells/mm2 by 24h. This had disappeared by 72h. In addition to resident Iba-1+ microglia in the ganglion cell layer and hyalocytes in the vitreous, there was an increased number of round Iba-1+ MHC Class II+ cells in the retina/vitreous of CpG ODN-treated eyes. This response was highly specific for CpG rich DNA, as similar topical exposure to (GpC) ODN did not induce any infiltration of inflammatory cells.

Conclusions: : Widespread intraocular inflammation can be induced by the application of CpG ODN to the injured cornea. These findings indicate that DNA released during microbial keratitis can stimulate an inflammatory response in the retina. Future studies will examine the specific cell types mediating TLR9 responses in the eye and the molecular basis for these observations.

Keywords: cornea: basic science • inflammation • microbial pathogenesis: experimental studies 
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