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D. Jo, J. Kim, K.-W. Kim, J. Kim, Y. Yu; Differential Expression of Matrix Metalloproteinases in Proliferation and Differentiation of Retinoblastoma Cells. Invest. Ophthalmol. Vis. Sci. 2010;51(13):2064. doi: https://doi.org/.
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The aim of this study was to investigate the expression of matrix metalloproteinase (MMP)-1, -3, and -7 in the retinoblastoma cells which were in the process of proliferation and differentiation.
Human retinoblastoma cells, SNUOT-Rb1, were incubated for 48 hours with basic fibroblast growth factors (bFGF) for proliferation and bovine serum albumin (BSA) for differentiation. After the incubation time, the cells were prepared for reverse transcriptase polymerase chain reaction (RT-PCR), and the levels of MMP-1, -3, and -7 were analyzed.
In proliferizing retinoblastoma cells, the levels of MMP-1 decreased, and those of MMP-3 and -7 increased than the control samples. During differentiation, the levels of MMP-1 remained the same, and those of MMP-3 and -7 were higher.
In the process of proliferation and differentiation of the retinoblastoma cells, the levels of various MMP molecules changed. These result suggest that for the treatment of retinoblastoma different strategies be established according to the types of MMP molecules.
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