April 2010
Volume 51, Issue 13
ARVO Annual Meeting Abstract  |   April 2010
The Distribution of Fgf-2/Perlecan and Collagen Iv in Bovine Lens Capsule as Quantified by Immunogold Labelling
Author Affiliations & Notes
  • W. Wu
    Biological and biomedical sciences,
    Durham University, Durham, United Kingdom
  • L. Bowen
    Department of Physics,
    Durham University, Durham, United Kingdom
  • R. Quinaln
    Biological and Biomedical Sciences,
    Durham University, Durham, United Kingdom
  • Footnotes
    Commercial Relationships  W. Wu, None; L. Bowen, None; R. Quinaln, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 2360. doi:
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      W. Wu, L. Bowen, R. Quinaln; The Distribution of Fgf-2/Perlecan and Collagen Iv in Bovine Lens Capsule as Quantified by Immunogold Labelling. Invest. Ophthalmol. Vis. Sci. 2010;51(13):2360.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : To investigated the distribution of FGF-2 and perlecan within the bovine lens capsule.

Methods: : Bovine lens epithelial cells were gently removed from the lens capsule by a curved tweezers. The lens capsules were fixed with a glutaraldehyde and formaldehyde combination, which preserved the epitopes for FGF-2, perlecan and collagen IV. The distribution of these three components was then measured by quantitative immunogold labelling of bovine lens capsule samples. These were flat mounted before being prepared for high resolution of a Hitachi SU70 scanning electron microscope. Images were collected and then tiled to document labelling across the entirety of the anterior and posterior lens capsule. Gold particles were counted using the ImageJ software. The data were tested for significance using the student T-test.

Results: : Both FGF-2 and perlecan epitopes were significantly more abundant in the equatorial region than either the anterior or posterior regions of the lens capsule. Interestingly, the posterior of the lens capsule was more heavily labelled than the anterior surface. Collagen IV epitopes were equally abundant in the anterior and equatorial regions, but were significantly decreased on the posterior lens capsule.

Conclusions: : The distribution of FGF-2 in bovine lens capsule fits the current hypothesis that there is an anterior-posterior gradient of FGF-2 available to lens epithelial cells. FGF-2 and its potential matrikine partner perlecan are concentrated in the equator. We suggest that the significant levels of FGF-2 and perlecan on the posterior lens capsule are a significant factor in the development of PCO.

Keywords: differentiation • extracellular matrix • growth factors/growth factor receptors 

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