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V. M. Bautista, Y. Garfias, N. Lopez-Espinosa, H. Mejia-Lopez, H. Perez-Cano, G. Mendoza-Hernandez; Differential Protein Expression in Human Pterygium. Invest. Ophthalmol. Vis. Sci. 2010;51(13):2405.
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Pterygium is one of the most frequent pathology in ophthalmology, and is a bening, fibrovascular lesion, originated from the bulbar conjunctiva. It is composed of epithelium and highly vascular, subepithelial, loose connective tissue. The ethiology of pterygium is not clearly understood; the most widely recognized origin factor is ultraviolet radiation. It has been proposed that pterygium and neoplasia have common features raising the possibility that pterygium is a neoplastic-like growth disorder. The aim of this study was to investigate the differences in protein expression between pterygium and healthy conjunctiva.
Four pterygium specimens were obtained together with healthy conjunctival tissue from the same eyes. Total proteins of pterygium and conjunctiva were analyzed in SDS-PAGE. This analysis showed protein bands expressed exclusively in pterygium samples at the range of 20-25 kDa. After that, two-dimensional electrophoresis was performed for the separation of total proteins; differential spots expressed in pterygium were identified and selected, to be analyzed by electrospray and mass spectrometry in tandem (ES-MS/MS).
These proteins were identified as peroxiredoxin 2, apolipoprotein AI and proapolipoprotein. RT-PCR showed that peroxiredoxin 2 and apolipoprotein AI were increased in pterygium compared to conjunctiva.
Peroxiredoxin-2 protects the cell against oxidative stress-induced apoptosis, and apolipoprotein AI has antiapoptotic effects and is related with carcinogenesis and progression. These results support that pterygium is a neoplastic-like growth disorder.
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