April 2010
Volume 51, Issue 13
ARVO Annual Meeting Abstract  |   April 2010
Laboratory Culture Positivity in Patients With Suspected Acanthamoeba Keratitis
Author Affiliations & Notes
  • M. L. Ribeiro
    Ophthalmology, UNIFESP, Sao Paulo, Brazil
  • P. V. Ferrari
    Ophthalmology, UNIFESP, Sao Paulo, Brazil
  • F. Hirai
    Ophthalmology, UNIFESP, Sao Paulo, Brazil
  • M. Zorat-Yu
    Ophthalmology, UNIFESP, Sao Paulo, Brazil
  • A. Hofling-Lima
    Ophthalmology, UNIFESP, Sao Paulo, Brazil
  • D. Freitas
    Ophthalmology, UNIFESP, Sao Paulo, Brazil
  • Footnotes
    Commercial Relationships  M.L. Ribeiro, None; P.V. Ferrari, None; F. Hirai, None; M. Zorat-Yu, None; A. Hofling-Lima, None; D. Freitas, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 2426. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      M. L. Ribeiro, P. V. Ferrari, F. Hirai, M. Zorat-Yu, A. Hofling-Lima, D. Freitas; Laboratory Culture Positivity in Patients With Suspected Acanthamoeba Keratitis. Invest. Ophthalmol. Vis. Sci. 2010;51(13):2426.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Purpose: : to investigate the positivity of microbiological results of corneal scrapings collected from patients suspected of having Acanthamoeba keratitis (AK)

Methods: : this was a cross-sectional analysis of records of patients who had suspected diagnosis of AK. Results from corneal scrapings sent to the Ocular Microbiology Laboratory - UNIFESP/EPM from September 1989 to July 2009 were reviewed. Definition of suspected diagnosis of AK: patients who had relevant risk factors such as duration of the lesion, personal history of contact lens use, symptoms or an ophthalmological examination suggesting an infectious keratitis in which AK could not be excluded.

Results: : Corneal scrapings were performed in 913 eyes of 884 patients with suspected diagnosis of AK and this condition was confirmed in 252 eyes (27.6%). In 76 eyes we observed concurrent growth of Acanthamoeba and bacteria and in 2 eyes (0,22%) there was fungal co-infection. Isolated bacterial infection was diagnosed in 337 eyes (36.9%) and isolated fungal infection in 14 eyes (1.5%).The most common agent isolated in all eyes was coagulase-negative Staphylococcus followed by Corynebacterium spp and Pseudomonas. The most common fungal isolated was Fusarium spp (7 eyes). There were 308 eyes (33,73%) free from infection.

Conclusions: : Positivity of laboratory culture was low among suspected cases in our service and there was a high prevalence of bacterial growth. The variation on lab culture indication, previous treatment with antibiotics and the similarity between clinical aspects on infectious keratitis indicates that lab cultures should be performed in our service in order to help with the investigation for an accurate diagnosis of such a challenging condition.

Keywords: Acanthamoeba • keratitis • clinical laboratory testing 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.