April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Development of a Standardised Method for the Quantified Determination of Contact Lens Disinfectant Efficacy Against Acanthamoeba
Author Affiliations & Notes
  • S. Kilvington
    Microbiology Research and Development, Abbott Medical Optics Inc., Santa Ana, California
  • A. Lam
    Microbiology Research and Development, Abbott Medical Optics Inc, Santa Ana, California
  • J. Lonnen
    Department of Infection, Immunity & Inflammation, University of Leicester, Leicester, United Kingdom
  • Footnotes
    Commercial Relationships  S. Kilvington, None; A. Lam, None; J. Lonnen, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 2428. doi:
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    • Get Citation

      S. Kilvington, A. Lam, J. Lonnen; Development of a Standardised Method for the Quantified Determination of Contact Lens Disinfectant Efficacy Against Acanthamoeba. Invest. Ophthalmol. Vis. Sci. 2010;51(13):2428.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Although standards exist for determining the efficacy of contact lens care solutions (CLCS) against bacteria and fungi (ISO 14729) none exist for Acanthamoeba. As such, conflicting findings on the effectiveness of CLCS against the organism have been reported. Here we describe a quantifiable, reproducible and reliable method for assessing the disinfection of Acanthamoeba by CLCS.

Methods: : A. castellanii

Results: : After a 6 hour contact time, the log reduction in trophozoite viability for the solutions were: MPS-1 (2.5 ±0.2 n=8), MPS-2 (1.8 ±0.2 n=8), MPS-3 (0.1 ±0.1 n=3), MPS-4 (2.7 ±0.2 n=8), PER-1 (3.8 ±0.2 n=8). For the cysts the values were: MPS-1 (2.9 ±0.3 n=3), MPS-2 (2.5 ±0.2 n=3), MPS-3 (0.7 ±0.1 n=3), MPS-4 (0.2 ±0.1 n=6) and PER-1 (2.7 ±0.3 n=3).

Conclusions: : The method is relatively simple to perform and enables the reproducible and quantifiable determination of CLCS efficacy against Acanthamoeba trophozoites and cysts. This will facilitate the introduction of a standardised and universal approach to Acanthamoeba disinfection testing in line with that prescribed by ISO 14729 for bacteria and fungi.

Keywords: Acanthamoeba • amoeba • contact lens 
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