April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Coinfection Bacteria-Fungus Causing Corneal Ulcer and Keratitis
Author Affiliations & Notes
  • H. Mejia-Lopez
    Research Unit, Institute of Ophthalmology, Mexico City, Mexico
  • H. J. Perez-Cano
    Research Unit, Institute of Ophthalmology, Mexico City, Mexico
  • C. Pantoja-Melendez
    Research Unit, Institute of Ophthalmology, Mexico City, Mexico
  • E. O. Medina- Hernández
    Research Unit, Institute of Ophthalmology, Mexico City, Mexico
  • J. J. Gutiérrez- Sereno
    Research Unit, Institute of Ophthalmology, Mexico City, Mexico
  • V. M. Bautista-de Lucio
    Research Unit, Institute of Ophthalmology, Mexico City, Mexico
  • Footnotes
    Commercial Relationships  H. Mejia-Lopez, None; H.J. Perez-Cano, None; C. Pantoja-Melendez, None; E.O. Medina- Hernández, None; J.J. Gutiérrez- Sereno, None; V.M. Bautista-de Lucio, None.
  • Footnotes
    Support  Institute of Ophthalmology "Conde de Valenciana", Mexico, D. F.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 2432. doi:https://doi.org/
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      H. Mejia-Lopez, H. J. Perez-Cano, C. Pantoja-Melendez, E. O. Medina- Hernández, J. J. Gutiérrez- Sereno, V. M. Bautista-de Lucio; Coinfection Bacteria-Fungus Causing Corneal Ulcer and Keratitis. Invest. Ophthalmol. Vis. Sci. 2010;51(13):2432. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Association between microorganisms is part of a successful evolutionary strategy they used to colonize other live organisms1, 2. Humans are often co-infected by multiple pathogens, a fact with great clinical relevance because it is related to the maintenance virulence and resistance to antimicrobial agents1,4. The aim of this study is to identify the association between Gram (+) and Gram (-) with fungi that affect the cornea.

Methods: : It is a retrospective study from January 2006 to July 2009 in samples from 234 patients with clinical diagnosis of corneal ulcer or keratitis. The identification of microorganisms was performed using PCR. To identify bacteria was use a nested PCR of 16S ribosomal gene, the first, amplifies a 1025 pb segment, the second, discriminate Gram (+) Gram (-). To identify fungi it was made a PCR amplified an 870 pb segment of 18S RNA gene, common to Candida sp, Aspergillus sp and Fusarium sp.Results and conclusions. It was found that patients with Gram (+) infection had 20% more at risk for fungal infection than those with Gram (-) (p> 0.5), however the X2 test indicated that there is not association between Gram (+) and Gram (-) and Fungi infections. In 2009 there was an increase 100% of Gram (+)- fungus coinfection in contrast to 2008.1. Proc Natl Acad Sci USA 1999;96:14547; 2. Science 2002;296:1077; 3. Proc Natl Acad Sci USA 1999;96:14547; 4. Nat Rev Microbiol 2004;2:552.

Keywords: keratitis • bacterial disease • fungal disease 
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