April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Pharmacokinetics of Antibody From Vitreous to Chorioretinal Region After Intravitreal Injection From Different Site
Author Affiliations & Notes
  • Y. Miura
    Department of Ophthalmology & Vis Sci,
    Nagasaki Univ Grad Sch of BioMed Sci, Nagasaki, Japan
  • M. Teshima
    Department of Hospital Pharmacy,
    Nagasaki Univ Grad Sch of BioMed Sci, Nagasaki, Japan
  • M. Uematsu
    Department of Ophthalmology & Vis Sci,
    Nagasaki Univ Grad Sch of BioMed Sci, Nagasaki, Japan
  • K. Suzuma
    Department of Ophthalmology & Vis Sci,
    Nagasaki Univ Grad Sch of BioMed Sci, Nagasaki, Japan
  • T. Kumagamiku
    Department of Ophthalmology & Vis Sci,
    Nagasaki Univ Grad Sch of BioMed Sci, Nagasaki, Japan
  • S. Hitoshi
    Department of Hospital Pharmacy,
    Nagasaki Univ Grad Sch of BioMed Sci, Nagasaki, Japan
  • T. Kitaoka
    Department of Ophthalmology & Vis Sci,
    Nagasaki Univ Grad Sch of BioMed Sci, Nagasaki, Japan
  • Footnotes
    Commercial Relationships  Y. Miura, None; M. Teshima, None; M. Uematsu, None; K. Suzuma, None; T. Kumagamiku, None; S. Hitoshi, None; T. Kitaoka, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 2441. doi:
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      Y. Miura, M. Teshima, M. Uematsu, K. Suzuma, T. Kumagamiku, S. Hitoshi, T. Kitaoka; Pharmacokinetics of Antibody From Vitreous to Chorioretinal Region After Intravitreal Injection From Different Site. Invest. Ophthalmol. Vis. Sci. 2010;51(13):2441.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Investigation of the difference in pharmacokinetics of antibody from vitreous to chorioretinal region after intravitreal injection from the different site of the vitreous cavity.

Methods: : For examination of pharmacokinetics of antibody from vitreous to chorioretinal region, 0.05 ml of the FITC labeled goat anti-mouse IgG (100 µg/ml) was injected from the superior pars plana to the vitreous cavity of the rabbit eye. An original intravitreal injection guide (Asian J Ophthalmol. 2006:8(3)156) was used to fix the tip of the injection needle in the superior-anterior vitreous (9.5mm posterior to center of cornea: anterior injection group), and posterior vitreous (19mm posterior to center of cornea : posterior injection group). 1 day, 4 days and 7 days after injection, the eyes were enucleated and frozen. The eyes were cut into three sections : superior-anterior, inferior-anterior and posterior sections, and after the vitreous was removed, the chorioretinal region was isolated. After homogenization, fluorescent intensity (excitation 485nm, emission 520 nm) was measured in each region to determine the concentration of the IgG.

Results: : In the posterior chorioretinal section, 1 day after the injection,the concentration of the IgG in the anterior injection group was 4.6 +/-0.9ng/1mg of the tissue, while 4.6+/-0.4ng/mg in the posterior injection group. 7 days after the injection, the concentration of the IgG in the anterior injection group was 4.0+/-0.7ng/mg, while 4.2+/-1.2ng/mg in the posterior injection group. There was no significant difference between the anterior injection group and the posterior injection group in the IgG concentrarion in the posterior chorioretinal region. In both groups, the concentration of IgG in posterior chorioretinal section was tended to decrease from day 1 to day 7 after intravitreal injection.

Conclusions: : In vitreous injection, it can be suggested that the chorioretinal concentration of IgG may be equal regardless of the site of the intravitreal injection.

Keywords: drug toxicity/drug effects • injection • vitreous 
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