April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Olfactory Ensheathing Cells Exert Neurotrophic Effects on Retinal Ganglion Cells in Culture and Promote Neurite Formation
Author Affiliations & Notes
  • S. Vijay
    NIHR Biomedical Research Centre for Ophthalmology, London, United Kingdom
    UCL Institute of Ophthalmology and Moorfields Eye Hospital, London, United Kingdom
  • A. H. Dahlmann-Noor
    NIHR Biomedical Research Centre for Ophthalmology, London, United Kingdom
    UCL Institute of Ophthalmology and Moorfields Eye Hospital, London, United Kingdom
  • R. Foxton
    UCL Institute of Ophthalmology and Moorfields Eye Hospital, London, United Kingdom
  • M. Bailly
    UCL Institute of Ophthalmology and Moorfields Eye Hospital, London, United Kingdom
  • P. T. Khaw
    NIHR Biomedical Research Centre for Ophthalmology, London, United Kingdom
    UCL Institute of Ophthalmology and Moorfields Eye Hospital, London, United Kingdom
  • Footnotes
    Commercial Relationships  S. Vijay, None; A.H. Dahlmann-Noor, None; R. Foxton, None; M. Bailly, None; P.T. Khaw, None.
  • Footnotes
    Support  Fight For Sight, The Helen Hamlyn Trust in memory of Paul Hamlyn, Dorothy Hodgkin Postgraduate Award, NIHR Biomedical Research Centre for Ophthalmology
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 2519. doi:
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      S. Vijay, A. H. Dahlmann-Noor, R. Foxton, M. Bailly, P. T. Khaw; Olfactory Ensheathing Cells Exert Neurotrophic Effects on Retinal Ganglion Cells in Culture and Promote Neurite Formation. Invest. Ophthalmol. Vis. Sci. 2010;51(13):2519.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose:
 

Olfactory ensheathing cells (OEC) promote neural repair in spinal cord lesions. We previously demonstrated that OEC transplanted into rat eyes associate with retinal ganglion cell (RGC) axons and optic nerve. Here we investigate the effects of OEC on RGC in co-culture.

 
Methods:
 

Three different OEC/RGC co-culture models: primary rat OEC with 1) RGC-5 cell line, 2) primary rat RGC, 3) retinal explants, in different matrix environments (on glass, in and on Matrigel/collagen matrices). Immunohistochemistry. Epifluorescence and confocal microscopy.

 
Results:
 

1) OEC form localised cell-cell contacts with the cell body and extensions of RGC-5 cells, both in two- and three-dimensional culture conditions. 2) In co-culture with primary RGC, OEC extensions form localised contacts with RGC processes (top left figure; red: RGC, green: OEC). 3) OEC cluster around retinal explants embedded in Matrigel and form arborizing structures (top right figure). OEC (bottom left figure; green) promote outgrowth of neurites (red) from retinal explant (blue). In the absence of OEC, no outgrowth is observed. High-resolution reconstruction of confocal z-stack (bottom right) demonstrates how RGC neurites (red) extend along scaffolds formed by OEC (green).

 
Conclusions:
 

OEC exert a neurotrophic effect on primary RGC and the RGC-5 cell line in culture and promote neurite outgrowth. Transplantation studies will investigate whether these effects may protect RGC axons in vivo and promote optic nerve rescue and regeneration.  

 
Keywords: retina: proximal (bipolar, amacrine, and ganglion cells) • neuroprotection • regeneration 
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