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I. Yoshioka, N. Ibaraki; Migration of Human Lens Epithelial Cells on the Vertical Wall Through Square Edge of Culture Dish. Invest. Ophthalmol. Vis. Sci. 2010;51(13):2616.
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To evaluate human lens epithelial (HLE) cells can migrate on the vertical wall through square edge of culture dish.
HLE cell line (SRA01/04) cells were cultured on the 25cm2 culture flask. To study the ability to migrate on the vertical wall through square edge, the cells on the vertical wall were observed by phase-contrast microscopy. To evaluate the growing rate on the vertical wall, the following procedures were studied. After reaching the confluent, the cells were scraped off only a little area remaining. And then, the culture medium was filled and the flask was stood for the flask bottom became on the vertical position. The cell growing rate was measured on the up-growth group and the down-growth group.
The cells could migrate on the vertical wall from the bottom of the flask. The square edge of culture flask was confirmed by scanning microscopy. At 3rd day of culture, the growing edge of up-growth and down-growth group reached 0.84 and 1.17 mm (p<0.005), respectively.
HLE cells migrate not only the bottom of the culture dish but also on the vertical wall. Only the square edge of the matrix does not prevent the posterior capsule opacification (PCO). The prevention of PCO by square edge of IOL may be affected by other factor from the square edge.
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