April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
The Roles of Plasminogen Activator Inhibitor-1 for the Pathogenesis of Atopic Cataracts
Author Affiliations & Notes
  • K. Hori
    Department of Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan
  • A. Matsuda
    Department of Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan
  • K. Imai
    Department of Ophthalmology, Kyoto Prefectural Univ of Med, Kyoto, Japan
  • K. Mori
    Department of Ophthalmology, Kyoto Prefectural Univ of Med, Kyoto, Japan
  • J. Hamuro
    Department of Ophthalmology, Kyoto Prefectural Univ of Med, Kyoto, Japan
  • S. Kinoshita
    Department of Ophthalmology, Kyoto Prefectural Univ of Med, Kyoto, Japan
  • A. Murakami
    Department of Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan
  • Footnotes
    Commercial Relationships  K. Hori, None; A. Matsuda, None; K. Imai, None; K. Mori, None; J. Hamuro, None; S. Kinoshita, None; A. Murakami, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 2620. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      K. Hori, A. Matsuda, K. Imai, K. Mori, J. Hamuro, S. Kinoshita, A. Murakami; The Roles of Plasminogen Activator Inhibitor-1 for the Pathogenesis of Atopic Cataracts. Invest. Ophthalmol. Vis. Sci. 2010;51(13):2620. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : We previously showed an association between interferon-gamma (IFN-γ) receptor polymorphisms and occurrence of atopic cataracts (IOVS 48:583-589, 2007). A recent study showed that IFN-γsignal is essential for PAI-1 induced post-operative fibrosis in abdomen (Nat Med. 14:437-441, 2008). In this study we further tried to clarify the roles of a fibrosis related protein, Plasminogen Activatior Inhibitor-1 (PAI-1), for the pathogenesis of atopic cataracts.

Methods: : Realtime-PCR analysis was carried out to compare the PAI-1 mRNA expression between the cDNA samples obtained during cataract surgery from the lens epithelium of atopic cataracts (n=10) and of senile cataracts(n=10). Cultured lens epithelial cells were stimulated by recombinant IFN-γ, and compared PAI-1 expression by real time PCR and Western blotting. Immunohistochemical analysis was carried out using the anterior capsular tissue obtained from atopic cataracts.

Results: : The relative expression of PAI-1 mRNA was significantly higher in the cDNA samples obtained from atopic cataracts compared to those from senile cataracts. Recombinant IFN-γ stimulation induced 4.6-fold higher PAI-1 mRNA expression in cultured lens epithelial cells at 24 hours after stimulation. PAI-1 positive immunostaining was observed at the subcapsular fibrosis of atopic cataracts.

Conclusions: : Our results suggested that IFN-γ- PAI-1 signaling cascade plays some roles for the pathophysiology of atopic cataracts.

Keywords: cataract • pathobiology • immunomodulation/immunoregulation 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×