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C.-K. Joo, H.-Y. L. Park, K.-M. Lee, M.-O. Park, J.-S. Choi; The Effect of NF-kB Inhibiting siRNA on Posterior Capsular Opacification. Invest. Ophthalmol. Vis. Sci. 2010;51(13):2626.
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The aim of this study was to investigate the effect of small interfering RNA (siRNA) of nuclear factor kappa B (NF-ΚB) on the development of posterior capsule opacification (PCO) in in vitro and in vivo study with rabbits
After application of p105 NF-ΚB siRNA to lens epithelial cells (LECs), western blot analysis to detect p105 and p50 NF-ΚB and scratch assay was done. In capsular bag model, LEC count and immunocytochemistry of p50 NF-ΚB of the posterior capsule was done. With fifteen New Zealand white rabbits, cataract surgery was performed using two sequence of p105 NF-ΚB siRNA. PCO grading was done by slit-lamp biomicroscopy and PCO grading program. After 1 month of surgery, eyes of the rabbit were enucleated and sections were obtained to observe posterior capsule and other ocular tissues under light microscope.
By applying p105 NF-ΚB siRNA to LECs, p105 NF-ΚB and p50 NF-ΚB were decreased by western blot analysis and migration of LECs was inhibited by scratch assay. In capsular bag model, cell count was significantly decreased and immunocytochemistry showed decrease of p50 NF-ΚB on the posterior capsule. In the in vivo study with rabbits, p105 NF-ΚB siRNA effectively decreased PCO with both slit-lamp examination and POCOman software assessment.
NF-ΚB seems to be related to the migration and proliferation of LECs. And using NF-ΚB siRNA shows to be effective in inhibiting the migration and proliferation of LECs in vitro and decreased PCO formation after cataract surgery in a rabbit.
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