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B. Tian, G. A. Heatley, M. S. Filla, P. L. Kaufman; Effect of H-7 on Secondary Cataract After Phacoemulsification in the Live Rabbit Eye. Invest. Ophthalmol. Vis. Sci. 2010;51(13):2635.
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To determine if the serine-threonine kinase inhibitor H-7 inhibits secondary cataract after extracapsular lens extraction by phacoemulsification in the live rabbit eye.
Eighteen albino rabbits underwent extracapsular lens extraction by phacoemulsification in one eye. The eye was treated with intravitreal H-7 (300 or 1,200µM; n= 6 or 5) or BSS (n=7) immediately after the surgery and twice weekly for 10 weeks. Each eye received slit lamp biomicroscopy once a week, during which the cornea status, intraocular inflammation (anterior chamber flare and cells) and posterior capsule opacification (PCO) were evaluated. The eye was then enucleated and the lens capsule was prepared, fixed and imaged. PCO was evaluated again on the isolated lens capsule under a phase microscope. Soemmering’s ring area (SRA) and the entire lens capsule area (the area measured along the capsule equator) were measured from capsule images on a computer and the percentage of SRA in the entire capsule area (PSRA) was calculated. Wet weight of the net capsule (WW) was determined on a balance.
No drug-induced corneal edema was observed. Intraocular inflammation in the H-7-treated eye, especially in the 1,200µM H-7-treated eye, was slightly more apparent than in the BSS-treated eye. No significant difference in PCO was observed in any comparisons. No significant differences in SRA, PSRA and WW were observed between the 300µM H-7-treated eye and the BSS-treated eye. However, differences in SRA (28.3±16.2 mm2 vs. 61.4±8.86 mm2), PSRA (33±20% vs. 65±15%) and WW (65.6±27.9 mg vs. 127±37.3 mg) between the 1,200 µM H-7-treated eye and the BSS-treated eye were statistically significant by the 2-tailed Mann-Whitney U test (P=0.005, P=0.018 and P=0.018 respectively) or by the 2-tailed unpaired t-test (P=0.001, P=0.01 and P=0.01 respectively).
This study indicates that 1,200µM intravitreal H-7 inhibits Soemmering’s ring formation in the live rabbit eye by ~50%, suggesting that agents that inhibit the actomyosin system in cells may prevent secondary cataract after phacoemulsification.
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