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B. J. Sanders, J. A. Canter, J. Cai, P. Sternberg; Age-Related Macular Degeneration (AMD) and Biomarkers of Oxidative Stress. Invest. Ophthalmol. Vis. Sci. 2010;51(13):2791.
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Isoprostane (IsoP) and isofuran (IsoF) are eicosaniods generated from peroxidation of arachidonic acid. Increased levels of F2-IsoPs have been reported in patients with various chronic diseases and are considered a reliable marker of oxidative stress, which is a contributing factor to the development of AMD. The goal of this study is to determine the association between plasma levels of IsoP and IsoF with disease status of AMD.
76 AMD patients and 70 age-matched control subjects were recruited at the Vanderbilt Eye Institute. The stage of AMD was documented by digital fundus photography and evaluated by a masked retina specialist. Plasma IsoP and IsoF were measured by thin layer chromatography (TLC) and mass spectrometry. Plasma thiol metabolites including cysteine and glutatione were measured by high performance liquid chromatography (HPLC).
The plasma level of IsoFs in control subjects had a median of 95 pg/ml with an interquartile range (IqR) of 51 to 151 pg/ml. In AMD patients, IsoFs had a median of 124 and with an IqR of 64 to 219. The difference was statistically significant (P<0.05 as determined by non-parametric Mann-Whitney U test. The levels of IsoPs were not different between the control and case group. There was no association between the thiol/disulfide redox status and other markers of lipid peroxidation.
Our data suggest that plasma biomarkers of lipid peroxidation are likely associated with AMD status. In future studies plasma IsoF may be used to monitor the therapeutic effects of antioxidant supplementation or other treatments that are effective in slowing the progression of AMD.
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