April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Effects of Selenium on Endothelial Cells of Mid-Term Stored Corneas in Rabbit Eyes
Author Affiliations & Notes
  • C. Qu
    Ophthalmology, Shanghai Tenth People's Hospital, Shanghai, China
  • W. Fang
    Ophthalmology, Shanghai Tenth People's Hospital, Shanghai, China
  • Footnotes
    Commercial Relationships  C. Qu, None; W. Fang, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 2827. doi:
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      C. Qu, W. Fang; Effects of Selenium on Endothelial Cells of Mid-Term Stored Corneas in Rabbit Eyes. Invest. Ophthalmol. Vis. Sci. 2010;51(13):2827.

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Abstract

Purpose: : To investigate the protective effects of selenium on endothelial cells of mid-term stored corneas in rabbit eyes.

Methods: : The rabbit corneas were stored differed in the irrigations of anterior chamber with M-K liquid, M-K liquid with sodium selenite (Na2SeO3) of 3uM, 5uM and 8uM. Enucleation was performed after 1 day, 3 days, 5 days, 7 days and 9 days respectively. The activity of GSH-Px in endothelial cells was estimated. Endothelial cells were actively stained by trypan blue. Morphological study was performed by specular microscope and electron microscope.

Results: : The activity of GSH-Px raised in the group with selenium compared with the group without selenium. The vital staining showed the endothelium survival rate in selenium group was higher. The percentage of the endothelial hexagonal cell was also higher, which is showed by specular microscope. The average cell density in selenium group was higher than the group without selenium after stored for 1 week. Under electronic microscope, the ultrastructural damages of corneal endothelial cells without selenium were more severe. The consecution of cell membrane was broken. Vacuoles in cytoplasm could be seen and the construction of organellae was lack of definition.

Conclusions: : The intermediate term storage media with selenium had significant differences in increasing the vitality of endothelial cells, and could prolong the time of corneal storage.

Keywords: cornea: endothelium • cornea: storage • cornea: basic science 
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