April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
The Efficacy of A ROCK Inhibitor Containing Corneal Storage Medium for Corneal Endothelial Cells
Author Affiliations & Notes
  • H. Takahashi
    Biomedical Engineering, Doshisha University, Kyotanabe, Japan
  • N. Koizumi
    Biomedical Engineering, Doshisha University, Kyotanabe, Japan
  • N. Okumura
    Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
  • M. Ueno
    Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
  • Y. Sakamoto
    Biomedical Engineering, Doshisha University, Kyotanabe, Japan
  • J. Hamuro
    Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
  • S. Kinoshita
    Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
  • Footnotes
    Commercial Relationships  H. Takahashi, None; N. Koizumi, None; N. Okumura, None; M. Ueno, None; Y. Sakamoto, None; J. Hamuro, None; S. Kinoshita, None.
  • Footnotes
    Support  The Japan Science and Technology Agency (Research for Promoting Technological Seeds # 10-090)
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 2829. doi:https://doi.org/
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    • Get Citation

      H. Takahashi, N. Koizumi, N. Okumura, M. Ueno, Y. Sakamoto, J. Hamuro, S. Kinoshita; The Efficacy of A ROCK Inhibitor Containing Corneal Storage Medium for Corneal Endothelial Cells. Invest. Ophthalmol. Vis. Sci. 2010;51(13):2829. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : The appropriate storage of donated human cornea is crucial for the surgical outcome following keratoplasty. We have previously demonstrated that Rho kinase (ROCK) inhibitor Y-27632 suppresses apoptosis in primate corneal endothelial cells (CECs) in vitro (Okumura, et al. IOVS 2009). The purpose of this current study was to demonstrate the usefulness of the Y-27632 as a supplement for corneal storage medium.

Methods: : Corneas obtained from Japanese white rabbits were stored in Optisol-GS® (Bausch & Lomb, Inc., Rochester, NY) corneal storage media supplemented by 10 µM of Y-27632 at 4°C for 14 days. Optisol-GS® without Y-27632 was used as the control. Annexin V-positive apoptotic cells and PI-positive dead cells in CECs were evaluated by immunohistochemistry. Hoechst 33258 was used for counterstaining.

Results: : The mean percentage of Annexin V-positive cells and PI-positive cells in the Y-27632 group (2.0±0.7% and 12.0±7.9%, respectively) were significantly suppressed compared with those in the control group (4.8±1.5% and 29.1±24.2%, respectively) (p<0.01, p<0.05) after 14 days.

Conclusions: : The results of this study demonstrate that Y-27632-supplemented corneal storage medium suppresses the apoptosis and cell death of CECs during corneal graft storage. We theorize that our results will also be applicable for human donor corneas in the clinical setting.

Keywords: cornea: storage • apoptosis/cell death • cell survival 
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