April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Predforte® but Not Restasis® Reduces Peak Corneal Haze After Photorefractive Keratectomy (PRK) in Rabbits
C. Nien Shy; K. J. Flynn; M. Chang; D. J. Brown; J. V. Jester
Author Affiliations & Notes
  • C. Nien Shy
    The Gavin Herbert Eye Institute, University of California, Irvine, Orange, California
  • K. J. Flynn
    The Gavin Herbert Eye Institute, University of California, Irvine, Orange, California
  • M. Chang
    The Gavin Herbert Eye Institute, University of California, Irvine, Orange, California
  • D. J. Brown
    The Gavin Herbert Eye Institute, University of California, Irvine, Orange, California
  • J. V. Jester
    The Gavin Herbert Eye Institute, University of California, Irvine, Orange, California
  • Footnotes
    Commercial Relationships  C. Nien Shy, None; K.J. Flynn, None; M. Chang, None; D.J. Brown, None; J.V. Jester, None.
  • Footnotes
    Support  NEI grants, EY07348 EY016663, The Discovery Eye Foundation, The Skirball Program in Molecular Ophthalmology, Support grant from Research to Prevent Blindness, Inc.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 2860. doi:
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      C. Nien Shy, K. J. Flynn, M. Chang, D. J. Brown, J. V. Jester; Predforte® but Not Restasis® Reduces Peak Corneal Haze After Photorefractive Keratectomy (PRK) in Rabbits. Invest. Ophthalmol. Vis. Sci. 2010;51(13):2860.

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Abstract

Purpose: : To evaluate the effects of topical cyclosporine (Restasis®) compared to steroids (PredForte®) on corneal haze, thickness and stromal cell density after PRK.

Methods: : Fifteen eyes(15 rabbits) received 9-D PRK. After the surgery, rabbits were divided into 3 groups and treated for 4 weeks with either PredForte®, Restasis® or used as control. PredForte® was administered 4 times a day during the first week and tapered over the next 3 weeks. Restasis® was administered twice a day for 4 weeks. Corneal haze was measured by in vivo confocal microscopy prior to and 2, 4, 6, 8, and 12 weeks postsurgery. At week 12, animals were sacrificed and corneas were evaluated for collagen organization by ex-vivo 2-photon second harmonic generation (SHG) and stromal cell density using DAPI staining of nuclei.

Results: : Corneal haze was significantly reduced in the PredForte® treated group compared to the Restasis® and control groups during the first 4 weeks after surgery (p< 0.002). At 6 weeks, haze slightly increased in the PredForte® group. For control and Restasis® groups, haze increased progressively reaching values peaked at 4 weeks after PRK and then slowly declined but remained elevated at 12 weeks. Stromal thickness was not statistically different at any time between groups (p=ns). Epithelial thickness was significantly reduced at day 7 after surgery in the PredForte® treated group (13.0µm compared to 35.4µm and 30.0µm for PredForte®, control and Restasis® respectively,p< 0.02) but no significant difference was detected 12 weeks after PRK between groups. Scar thickness measured at 12 weeks by SHG was not significantly different between the 3 groups (57.64µm, 55.58µm and 54.21µm for control, Restasis® and PredForte® respectively,p=ns). Stromal cell density count at 12 weeks had a tendency to be lower in the Pred Forte® treated group (43,723 vs 63,077 and 67,080 for Pred Forte®, control and Restasis® respectively,p=ns).

Conclusions: : Restasis® has no effect on wound healing following PRK while PredForte® significantly reduces peak corneal haze but has no effect on long-term corneal fibrosis after discontinuation of the drug.

Keywords: wound healing • refractive surgery • cornea: stroma and keratocytes 
© 2010, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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