April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Bacterial Infiltration to the Lens Capsule in Experimental Endophthalmitis
Author Affiliations & Notes
  • K. Kakisu
    1st Dept of Ophthalmology, Toho University, Ohta-ku, Japan
  • S. Kobayakawa
    1st Dept of Ophthalmology, Toho University, Ohta-ku, Japan
  • T. Tochikubo
    1st Ophthalmology, Toho University, Ohta-Ku, Japan
  • Footnotes
    Commercial Relationships  K. Kakisu, None; S. Kobayakawa, None; T. Tochikubo, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 2898. doi:
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      K. Kakisu, S. Kobayakawa, T. Tochikubo; Bacterial Infiltration to the Lens Capsule in Experimental Endophthalmitis. Invest. Ophthalmol. Vis. Sci. 2010;51(13):2898.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To investigate the influence of bacterial proliferation to the lens capsule in removed pig eyes (ex vivo) and rabbit eyes (in vivo).

Methods: : Clinically isolated methicillin-resistant Staphylococcus aureus (MRSA, UA-1) and Staphylococcus epidermidis (ATCC 35984) were used. Small quantities of the culture were subcultured on the BHI broth overnight at 37°C. Then, cultures were kept frozen at -75°C in MicrobankTM (PRO-LAB Diagnostics, Toronto, Canada). Twelve Japanese albino rabbits and twenty-four fresh removed pig eyes were also used. Phacoemulsification was performed in removed pig eyes and rabbit eyes under anesthesia. The incision size was increased to approximately 5.5 mm at the end of surgery. Two beads from MicrobankTM tube were inserted into the capsular bag for each pig and rabbit eyes. Wound closure was achieved with a suture. Those operated pig eyes were kept in centrifuge tubes with 10ml PBS at 37°C. After incubation for 24 and 48 hours, those pig eyes were fixed with 10% formalin. The rabbits were euthanized at 24 hours postoperatively, and those eyes were enucleated for observation. Observations were performed with hematoxylin-eosin stain.

Results: : The starting inoculum was log106.6 CFU/eye, the bacterial population was log107.6 CFU/ml in removed pig eyes at 24 hours incubation. The destruction of ocular tissue in pig eyes was ongoing at 48 hours incubation. As for the S.epidermidis, those bacteria were aggregated on the surface of lens capsule, while MRSA were infiltrated to the anterior and posterior lens capsule at 24 hours incubation. In the rabbit eyes, also MRSA were infiltrated to the lens capsule at 24 hours postoperatively.

Conclusions: : Pathological changes were noted in the lens capsule of eyes infected with MRSA at 24 hours incubation. MRSA may spread to the vitreous cavity and anterior chamber passing through the lens capsule in endophthalmitis.CR: None

Keywords: endophthalmitis • cataract • microbial pathogenesis: experimental studies 
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