April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Comparative Efficacy of Besifloxacin in a Prophylaxis Model of Penicillin-Resistant Streptococcus pneumoniae Rabbit Endophthalmitis
Author Affiliations & Notes
  • E. W. Norcross
    Microbiology, University of Mississippi Medical Center, Jackson, Mississippi
  • M. E. Sanders
    Microbiology, University of Mississippi Medical Center, Jackson, Mississippi
  • Q. C. Moore, III
    Microbiology, University of Mississippi Medical Center, Jackson, Mississippi
  • C. M. Sanfilippo
    Microbiology,
    Bausch & Lomb, Inc., Rochester, New York
  • C. K. Hesje
    Microbiology,
    Bausch & Lomb, Inc., Rochester, New York
  • A. Shafiee
    Pharmacology,
    Bausch & Lomb, Inc., Rochester, New York
  • M. E. Marquart
    Microbiology, University of Mississippi Medical Center, Jackson, Mississippi
  • Footnotes
    Commercial Relationships  E.W. Norcross, None; M.E. Sanders, None; Q.C. Moore, III, None; C.M. Sanfilippo, Bausch & Lomb, Inc., E; C.K. Hesje, Bausch & Lomb, Inc., E; A. Shafiee, Bausch & Lomb, Inc., E; M.E. Marquart, Bausch & Lomb, Inc., C.
  • Footnotes
    Support  Bausch & Lomb, Inc.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 2901. doi:
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      E. W. Norcross, M. E. Sanders, Q. C. Moore, III, C. M. Sanfilippo, C. K. Hesje, A. Shafiee, M. E. Marquart; Comparative Efficacy of Besifloxacin in a Prophylaxis Model of Penicillin-Resistant Streptococcus pneumoniae Rabbit Endophthalmitis. Invest. Ophthalmol. Vis. Sci. 2010;51(13):2901.

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Abstract

Purpose: : Fluoroquinolones show broad spectrum antibacterial activity and are used to treat a wide range of bacterial infections. However, studies have shown that pneumococcal strains with penicillin resistance were more likely to also show resistance to fluoroquinolones. To date there have been no in vivo studies regarding the efficacy of fluoroquinolones against penicillin-resistant S. pneumoniae endophthalmitis. Therefore, the aim of the study was to study the efficacy of topical administration of gatifloxacin (0.3%), moxifloxacin (0.5%) ophthalmic solutions, and besifloxacin (0.6%) ophthalmic suspension in the prophylaxis and treatment of penicillin-resistant pneumococcal endophthalmitis.

Methods: : Four groups of New Zealand white rabbits were topically treated with gatifloxacin, moxifloxacin, besifloxacin, and PBS at the following time points: 60, 45, 30, and 15 minutes prior to infection, immediately after infection, and then 6, 12, 18, and 24 hours post-infection. Penicillin-resistant Streptococcus pneumoniae (106 CFU in 50 µl) were injected intracamerally. The severity of the infection was assessed by two masked observers 24 hours post-infection by clinical examination. Thereafter, aqueous and vitreous samples were collected, diluted, and plated to determine bacterial load (i.e., recovered CFU).

Results: : The three fluoroquinolones reduced clinical scores and bacterial load in the aqueous humor when compared to the PBS-treated eyes. No statistically significant differences were observed among all the fluoroquinolone-treated groups. Additionally, there was no statistically significant difference in bacterial load in the vitreous humor among the fluoroquinolones or when compared to PBS group.

Conclusions: : Besifloxacin is as effective as moxifloxacin and gatifloxacin in a rabbit model for the prophylaxis and treatment for penicillin-resistant S. pneumoniae endophthalmitis after topical administration.

Keywords: antibiotics/antifungals/antiparasitics • endophthalmitis • bacterial disease 
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