April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
The Evaluation of Intracameral Injection of Levofloxacin in the Prophylaxis of Postoperative Enterococcus Faecalis Endophthalmitis
T. Uda; Y. Tasaka; S. Kawasaki; T. Mito; T. Suzuki; T. Uno; Y. Ike; Y. Ohashi
Author Affiliations & Notes
  • T. Uda
    Department of Ophthalmology, Ehime university School of Medicine, Ehime, Japan
  • Y. Tasaka
    Department of Ophthalmology, Ehime University School of Medicine, Ehime, Japan
  • S. Kawasaki
    Department of Ophthalmology, Ehime University School of Medicine, Ehime, Japan
  • T. Mito
    Department of Ophthalmology, Ehime University School of Medicine, Ehime, Japan
  • T. Suzuki
    Department of Ophthalmology, Ehime University School of Medicine, Ehime, Japan
  • T. Uno
    Department of Ophthalmology, Ehime University School of Medicine, Ehime, Japan
  • Y. Ike
    Department of Bacteriology, Gunma University School of Medicine, Maebashi, Gunma, Japan
  • Y. Ohashi
    Department of Ophthalmology, Ehime University School of Medicine, Ehime, Japan
  • Footnotes
    Commercial Relationships  T. Uda, None; Y. Tasaka, None; S. Kawasaki, None; T. Mito, None; T. Suzuki, None; T. Uno, None; Y. Ike, None; Y. Ohashi, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 2902. doi:
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      T. Uda, Y. Tasaka, S. Kawasaki, T. Mito, T. Suzuki, T. Uno, Y. Ike, Y. Ohashi; The Evaluation of Intracameral Injection of Levofloxacin in the Prophylaxis of Postoperative Enterococcus Faecalis Endophthalmitis. Invest. Ophthalmol. Vis. Sci. 2010;51(13):2902.

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Abstract

Purpose: : Rupture of the lens capsule is known to be a risk factor for endophthalmitis and suppurative posterior infection. Intracameral injection of antibiotics should be one of option for prevention of endophthalmitis in high risk cases having rupture of the lens capsule. This study was designed to evaluate intracameral injection of levofloxacin (LVFX) for prophylaxis of endophthalmitis using an aphakic rabbit model.

Methods: : After the lens removal by phacoemulsification with intentional rupture of lens capsule, LVFX (500µg/0.1ml) was injected into anterior chamber. The eyes were enucleated 2 hour after injection, and the concentration of LVFX in vitreous humor and aqueous humor was estimated using high performance liquid chromatography. To determine the efficacy of LVFX intracameral injection for prevention of endophthalmitis, aphakic eyes inoculated with 1×104colony-forming units (CFU) of E. faecalis OG1S (secreted protease-positive) into anterior chamber having rupture of lens capsule were used. After bacterial inoculation, intracameral injection of LVFX (500µg/0.1ml) or saline was performed. Changes in electroretinography (ERG) and numbers of organisms were determined and compared throughout the infection by 48 hour.

Results: : Mean ± SD concentrations of LVFX 2 hour after intracameral injection were as follows: aqueous humor (129±53 µg/ml) and vitreous humor (anterior part; 45.2±29.8µg/ml, mid part; 63±63.9µg/ml, posterior part; 74.8±80.8µg/ml). Eyes treated with saline resulted in a significantly greater reduction of B-wave amplitude of ERG at 48 hour than eyes treated with LVFX. (test/control eye ratio; saline:12.4±7.9 %, LVFX: 124.3±29.8 %; P<0.01) The number of bacteria recovered from vitreous humor in the eye treated with LVFX at 48 hour was significantly less than eyes treated with saline. (saline: 8.2±0.6 log10 CFU/tissue, LVFX: 5.1±1.3 log10 CFU/tissue, P<0.01)

Conclusions: : Intracameral injection of LVFX into eyes with rupture of lens capsule can penetrate deeply and was effective in preventing endophthalmitis caused by E. faecalis.

Keywords: endophthalmitis • antibiotics/antifungals/antiparasitics • cataract 
© 2010, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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