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I. Berra, G. Zapata, A. Berra; Effect of Dietary Fish Oil Upon the Development of Murine Herpetic Retinal Necrosis. Invest. Ophthalmol. Vis. Sci. 2010;51(13):2906.
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To examine the effects of diets enriched with n-3 and n-6 polyunsaturatedfatty acids (PUFAs) in a murine model of herpetic chorioretinitis.
BALB/c mice were fed on high-fat diets which contained Menhadenoil (rich in n-3 PUFAs) (diet 1), Safflower oil (rich in n-6PUFAs) (diet 2), or Corn oil as control(diet 3), 14 days previouslyand 12 days following anterior chamber (AC) HSV-1 inoculation.After 14 days of treatment and before infection, splenic cellproliferation was evaluated as well as cytokines synthesis onculture supernatants.Development of chorioretinitis and encephalitiswas followed-up by clinical and histological signs. On day 12p.i. mice were sacrificed. Both eyes and brain were obtainedfor viral titration and RT-PCR analysis.
Mice fed on Menhaden oil experimented a decreased synthesisof TNF-α, IFN-γ, IL-2 and IL-10 in splenic cells isolated andstimulated by Con-A. Post HSV-1 inoculation, mice fed on Menhadenoil showed clinical and histological signs of chorioretinitisin both eyes and encephalitis. Moreover, high titers of viruswere recovered in ocular and brain tissue homogenates.
Our findings indicate that mice fed on fish oil have impairedTh-1 immune respons which could lead to increased virus titersand consequent development of encephalitis and chorioretinisin both eyes. We also conclude that small but detectable amountsof virus (1x10(4) PFU/ml) could be present at uninoculated eyeswithout clinical and histopathological evidence of retinitis.It suggests that the triggering of retinal injury should requirea critical threshold of virus.
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