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M. Ogawa, S. Sugita, N. Shimizu, T. Morio, M. Mochizuki; Use of Human Herpes Virus (HHV) PCR Assays to Detect Viral DNA in Ocular Fluids of Patients With Herpetic Eye Diseases. Invest. Ophthalmol. Vis. Sci. 2010;51(13):2907.
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© ARVO (1962-2015); The Authors (2016-present)
To measure the genomic DNA of human herpes viruses (HHV) in the ocular fluids and to analyze the clinical relevance of HHV in clinically suspected herpetic eye diseases.
After informed consent was obtained, a total of 184 ocular fluid samples (aqueous humor and/or vitreous fluid samples) were collected from patients with clinically suspected herpetic eye diseases, i.e. unilateral involvement of keratouveitis, corneal endotheliitis, anterior uveitis with ocular hypertension and mutton-fat keratic precipitates, and necrotic retinitis. The samples were assayed for HHV-DNA (HHV-1~HHV-8) by using two different polymerase chain reaction (PCR) assays: qualitative multiplex PCR followed by quantitative real-time PCR. As the controls, intraocular fluid samples of non-infectious uveitis (e.g., sarcoidosis) and non-uveitic ocular disease (e.g., retinal detachment) were examined.
Genomic DNA of HHV (HHV-1~HHV-8) were detected in 103 (56%) of 184 ocular fluid samples (155 aqueous humor and 29 vitreous fluid) with high copy numbers of HHV DNA (>103-108 levels). HHV-DNA was positive in keratouveitis in 71% (5/7 samples), corneal endotheliitis in 33% (7/21), anterior uveitis with ocular hypertension in 39% (37/96), and necrotic retinitis in 87% (54/62). On the other hand, HHV DNA was not detected in any samples (n=50) of patients with non-infectious uveitis or non-uveitic ocular diseases.
HHV-DNA is frequently detected in ocular fluids from patients clinically suspected herpetic eye diseases. Thus, examination of PCR using ocular fluids is useful to make a diagnosis in such cases.
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