April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Use of Human Herpes Virus (HHV) PCR Assays to Detect Viral DNA in Ocular Fluids of Patients With Herpetic Eye Diseases
Author Affiliations & Notes
  • M. Ogawa
    Ophthalmology & Visual Science, tokyo medical and dental university, tokyo, Japan
  • S. Sugita
    Ophthalmology & Visual Science,
    Tokyo Medical and Dental University, Tokyo, Japan
  • N. Shimizu
    Virology, Medical Research Institute,
    Tokyo Medical and Dental University, Tokyo, Japan
  • T. Morio
    Center for Cell Therapy,
    Tokyo Medical and Dental University, Tokyo, Japan
  • M. Mochizuki
    Ophthalmology & Visual Science,
    Tokyo Medical and Dental University, Tokyo, Japan
  • Footnotes
    Commercial Relationships  M. Ogawa, None; S. Sugita, None; N. Shimizu, None; T. Morio, None; M. Mochizuki, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 2907. doi:https://doi.org/
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      M. Ogawa, S. Sugita, N. Shimizu, T. Morio, M. Mochizuki; Use of Human Herpes Virus (HHV) PCR Assays to Detect Viral DNA in Ocular Fluids of Patients With Herpetic Eye Diseases. Invest. Ophthalmol. Vis. Sci. 2010;51(13):2907. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To measure the genomic DNA of human herpes viruses (HHV) in the ocular fluids and to analyze the clinical relevance of HHV in clinically suspected herpetic eye diseases.

Methods: : After informed consent was obtained, a total of 184 ocular fluid samples (aqueous humor and/or vitreous fluid samples) were collected from patients with clinically suspected herpetic eye diseases, i.e. unilateral involvement of keratouveitis, corneal endotheliitis, anterior uveitis with ocular hypertension and mutton-fat keratic precipitates, and necrotic retinitis. The samples were assayed for HHV-DNA (HHV-1~HHV-8) by using two different polymerase chain reaction (PCR) assays: qualitative multiplex PCR followed by quantitative real-time PCR. As the controls, intraocular fluid samples of non-infectious uveitis (e.g., sarcoidosis) and non-uveitic ocular disease (e.g., retinal detachment) were examined.

Results: : Genomic DNA of HHV (HHV-1~HHV-8) were detected in 103 (56%) of 184 ocular fluid samples (155 aqueous humor and 29 vitreous fluid) with high copy numbers of HHV DNA (>103-108 levels). HHV-DNA was positive in keratouveitis in 71% (5/7 samples), corneal endotheliitis in 33% (7/21), anterior uveitis with ocular hypertension in 39% (37/96), and necrotic retinitis in 87% (54/62). On the other hand, HHV DNA was not detected in any samples (n=50) of patients with non-infectious uveitis or non-uveitic ocular diseases.

Conclusions: : HHV-DNA is frequently detected in ocular fluids from patients clinically suspected herpetic eye diseases. Thus, examination of PCR using ocular fluids is useful to make a diagnosis in such cases.

Keywords: herpes simplex virus • inflammation • inflammation 
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