April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Multielectrode Recording of Responses From Retinal Ganglion Cells to Photic Stimuli of Variable Area - Cell Spatial Distribution
Author Affiliations & Notes
  • O. R. Ziv
    Ctr. for Innovative Visual Rehabilitation, VA Boston Healthcare System, Boston, Massachusetts
    Rle, Massachusetts In stitute of Technology, Cambridge, Massachusetts
  • R. J. Jensen
    Ctr. for Innovative Visual Rehabilitation, VA Boston Healthcare System, Boston, Massachusetts
  • J. F. Rizzo
    Ctr. for Innovative Visual Rehabilitation, VA Boston Healthcare System, Boston, Massachusetts
    Ophthalmology, Mass Eye & Ear Infirmary, Boston, Massachusetts
  • Footnotes
    Commercial Relationships  O.R. Ziv, None; R.J. Jensen, None; J.F. Rizzo, None.
  • Footnotes
    Support  Department of Veterans Affairs, Veterans Health Administration, RR & D Service
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 3016. doi:
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      O. R. Ziv, R. J. Jensen, J. F. Rizzo; Multielectrode Recording of Responses From Retinal Ganglion Cells to Photic Stimuli of Variable Area - Cell Spatial Distribution. Invest. Ophthalmol. Vis. Sci. 2010;51(13):3016.

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Abstract

Purpose: : This work is related to the efforts of the Boston Retinal Implant Project to develop a sub-retinal prosthesis to restore vision to the blind. The specific purpose of this presentation is to demonstrate a method to better understand the population responses of retinal ganglion cells (RGCs) to various sizes of photic stimuli using a multielectrode array. The goal is to better understand the responses of RGCs to light stimulation in order to mimic these responses electrically by a retinal prosthesis.

Methods: : Extracellular recordings of action potentials of RGCs were made in superfused New Zealand White rabbit retinas using a multielectrode array. The retina was stimulated by flashes of light at different diameters (100µm, 200µm, 300µm, and 500µm). The photic stimulus duration was 0.5sec. The data collected from 312 different recordings (78 stimulations times 4 spot sizes) were analyzed by sorting the action potentials and measuring temporal (on-brief, off-brief, on-sustained, off-sustained, and on-off type responses) and spatial (relative distance) parameters.

Results: : Recordings were obtained from variety of RGCs. The responses of these cells to light flashes included onset, offset, or both responses for cells at different distances from the edge of the stimulus. The stimulation produced responses at different distances from the stimulating light spot. Spatial distribution maps show different distribution to the different types of cells. The maps show the numbers and relative locations of each cell type at each distance from the stimulus. The different behavior between cell types is clearly evident from the maps.

Conclusions: : RGCs responds to a photic stimulus up to certain distances from the edge of the light spot. Different cell types have different spatial distributions. The relative number of responding cells differs according to the cell type.

Keywords: retina • ganglion cells • anatomy 
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