April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Assessment of in vivo Ocular Toxicity of Nitric Oxide Donors Compounds
Author Affiliations & Notes
  • A. J. Cariello
    Ophthalmology,
    Sao Paulo Fed Univ, Sao Paulo, Brazil
  • G. F. P. Souza
    Chemistry, State University of Campinas, Campinas, Brazil
  • M. S. Lowen
    Pathology,
    Sao Paulo Fed Univ, Sao Paulo, Brazil
  • S. A. Nakayama
    Ophthalmology,
    Sao Paulo Fed Univ, Sao Paulo, Brazil
  • M. G. Oliveira
    Chemistry, State University of Campinas, Campinas, Brazil
  • A. L. Hofling-Lima
    Ophthalmology,
    Sao Paulo Fed Univ, Sao Paulo, Brazil
  • Footnotes
    Commercial Relationships  A.J. Cariello, None; G.F.P. Souza, None; M.S. Lowen, None; S.A. Nakayama, None; M.G. Oliveira, None; A.L. Hofling-Lima, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 3123. doi:
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      A. J. Cariello, G. F. P. Souza, M. S. Lowen, S. A. Nakayama, M. G. Oliveira, A. L. Hofling-Lima; Assessment of in vivo Ocular Toxicity of Nitric Oxide Donors Compounds. Invest. Ophthalmol. Vis. Sci. 2010;51(13):3123.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To evaluate the ocular toxicity of two nitric oxide donors: S-nitrosoglutathione (GSNO) and S-nitroso-N-acetylcysteine (SNAC) prepared in a methylcellulose matrix by using an in vivo animal model.

Methods: : twenty albino rabbits were enrolled in this study. All animals underwent clinical slit lamp examination. The animals were randomized into 4 groups of 5 animals: Group 1 (GSNO1), 0.15ml of GSNO at a concentration of 1mM prepared in a methylcellulose matrix was instilled onto the right eye. The left eye received the same volume of methylcellulose matrix (control). Group 2 (GSNO10), Group 3 (SNAC1) and Group 4 (SNAC10), the same procedure was performed with GSNO at concentration of 10mM and SNAC at concentrations of 1 and 10mM, respectively. After 30 minutes, 24 and 48 hours all the animals underwent a clinical evaluation and were scored according to Draize eye test score. The eyes were also analyzed histologically.

Results: : There was no difference between control and treatment eye according Draize eye test score in all groups (p<0.05). There was no evidence of tissue toxicity in the histological analysis in all animals.

Conclusions: : GSNO and SNAC at concentration of 1 and 10 mM are not considered ocular irritating compounds. These compounds have potential to be used as therapeutic agents in ophtalmology.

Keywords: drug toxicity/drug effects • nitric oxide • ocular irritancy/toxicity testing 
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