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A. J. Cariello, G. F. P. Souza, M. S. Lowen, S. A. Nakayama, M. G. Oliveira, A. L. Hofling-Lima; Assessment of in vivo Ocular Toxicity of Nitric Oxide Donors Compounds. Invest. Ophthalmol. Vis. Sci. 2010;51(13):3123.
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To evaluate the ocular toxicity of two nitric oxide donors: S-nitrosoglutathione (GSNO) and S-nitroso-N-acetylcysteine (SNAC) prepared in a methylcellulose matrix by using an in vivo animal model.
twenty albino rabbits were enrolled in this study. All animals underwent clinical slit lamp examination. The animals were randomized into 4 groups of 5 animals: Group 1 (GSNO1), 0.15ml of GSNO at a concentration of 1mM prepared in a methylcellulose matrix was instilled onto the right eye. The left eye received the same volume of methylcellulose matrix (control). Group 2 (GSNO10), Group 3 (SNAC1) and Group 4 (SNAC10), the same procedure was performed with GSNO at concentration of 10mM and SNAC at concentrations of 1 and 10mM, respectively. After 30 minutes, 24 and 48 hours all the animals underwent a clinical evaluation and were scored according to Draize eye test score. The eyes were also analyzed histologically.
There was no difference between control and treatment eye according Draize eye test score in all groups (p<0.05). There was no evidence of tissue toxicity in the histological analysis in all animals.
GSNO and SNAC at concentration of 1 and 10 mM are not considered ocular irritating compounds. These compounds have potential to be used as therapeutic agents in ophtalmology.
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