April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Outgrowth Endothelial Cells Potential for Reversing Ischaemic Retinopathy
Author Affiliations & Notes
  • A. W. Stitt
    Centre for Vision & Vascular Science, Queens University Belfast, Belfast, United Kingdom
  • R. J. Medina
    Centre for Vision & Vascular Science, Queens University Belfast, Belfast, United Kingdom
  • C. L. O'Neill
    Centre for Vision & Vascular Science, Queens University Belfast, Belfast, United Kingdom
  • M. W. Humphreys
    Northern Ireland Regional Genetics Centre, Belfast Health and Social Care Trust, Belfast, United Kingdom
  • T. A. Gardiner
    Centre for Vision & Vascular Science, Queens University Belfast, Belfast, United Kingdom
  • Footnotes
    Commercial Relationships  A.W. Stitt, None; R.J. Medina, None; C.L. O'Neill, None; M.W. Humphreys, None; T.A. Gardiner, None.
  • Footnotes
    Support  JDRF, MRC, GDBA
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 3155. doi:
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      A. W. Stitt, R. J. Medina, C. L. O'Neill, M. W. Humphreys, T. A. Gardiner; Outgrowth Endothelial Cells Potential for Reversing Ischaemic Retinopathy. Invest. Ophthalmol. Vis. Sci. 2010;51(13):3155.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : The purpose of this study was to isolate endothelial progenitor cells from human peripheral blood, characterise a distinct endothelial progenitor cell population named Outgrowth Endothelial Cells (OECs) and establish their potential as a novel cell therapy for ischaemic retinopathy.

Methods: : OECs were isolated and characterised using immunophenotyping, genome-wide transcriptional profiling, and multiple in vitro functional assays to assess interaction with retinal capillary endothelial cells and angiogenic activity. OECs were delivered intravitreally in a mouse model of ischaemic retinopathy, and flat mounted retinas were examined using confocal microscopy.

Results: : Our data indicate that OECs are committed to an endothelial lineage, have significant proliferative, and de novo tubulogenic potential. Furthermore, OECs are able to closely interact with endothelial cells through adherens and tight junctions, and integrate into retinal vascular networks in vitro. Using a murine model of retinal ischaemia, it is demonstrated that OECs directly incorporate into the resident vasculature, significantly decreasing avascular areas, concomitantly increasing normovascular areas and preventing pathologic pre-retinal neovascularisation.

Conclusions: : OECs have potential as therapeutic cells to vascularise the ischaemic retina and prevent sight-threatening pathology.

Keywords: ischemia • retina • retinal neovascularization 
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