April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Mechanism of Stem Cells in Rescuing Vision in a Rodent Model of Retinal Degeneration
Author Affiliations & Notes
  • S. Wang
    Ophthalmology, Casey Eye Institute, OHSU, Portland, Oregon
  • B. Cottam
    Ophthalmology, Casey Eye Institute, OHSU, Portland, Oregon
  • B. Lu
    Ophthalmology, Casey Eye Institute, OHSU, Portland, Oregon
  • C. W. Morgans
    Ophthalmology, Casey Eye Institute, OHSU, Portland, Oregon
  • T. J. McFarland
    Ophthalmology, Casey Eye Institute, OHSU, Portland, Oregon
  • B. Appukuttan
    Ophthalmology, Casey Eye Institute, OHSU, Portland, Oregon
  • E. E. Capowski
    Waisman Center, University of Wisconsin-Madison, Madison, Wisconsin
  • D. M. Gamm
    Ophthalmology and Visual Sciences, Univ of Wisconsin-Madison, Madison, Wisconsin
  • R. D. Lund
    Ophthalmology, Casey Eye Institute, OHSU, Portland, Oregon
  • Footnotes
    Commercial Relationships  S. Wang, None; B. Cottam, None; B. Lu, None; C.W. Morgans, None; T.J. McFarland, None; B. Appukuttan, None; E.E. Capowski, None; D.M. Gamm, None; R.D. Lund, None.
  • Footnotes
    Support  FFB, RPB
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 3157. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      S. Wang, B. Cottam, B. Lu, C. W. Morgans, T. J. McFarland, B. Appukuttan, E. E. Capowski, D. M. Gamm, R. D. Lund; Mechanism of Stem Cells in Rescuing Vision in a Rodent Model of Retinal Degeneration. Invest. Ophthalmol. Vis. Sci. 2010;51(13):3157. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : Cell-based therapy has been shown to be effective in slowing down the progression of retinal degeneration in animal models. However, the molecular mechanisms underlying vision rescue are unclear. It is likely that both the injected cells and the host retina undergo molecular changes post-transplant. Here we study changes in expression of growth factor genes by human progenitor cells before and after injection into the eye in a rodent model of retinal degeneration, as well as changes in gene expression in host retinal neurons following the injection.

Methods: : Human forebrain derived progenitor cells (hNPCctx) were injected into the subretinal space of RCS rats at postnatal day (P) 21-23. The efficacy of injected cells was examined by measurements of visual acuity. Retinal tissues and injected hNPCctx were collected at P35, P60 and P90 using laser capture microdissection (LCM). RNA from hNPCctx and retinal tissues were isolated and examined for expression of trophic factors by polymerase chain reaction and immunohistochemistry.

Results: : Subretinal injection of hNPCctx produced substantial rescue morphologically and functionally. Trophic factors released by human donor cells, including IGF and BDNF, were detected both in vitro and in vivo. However, at P90, BDNF was no longer detected. The host retina expressed Apolipoprotein E in areas close to the donor cell distribution.

Conclusions: : This study demonstrates that vision rescue by injected hNPCctx is correlated with the expression of trophic factors released by both donor cells and the host retina. The cellular localization of these factors in the host retina is under investigation.

Keywords: retinal degenerations: cell biology • gene/expression • transplantation 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×