April 2010
Volume 51, Issue 13
ARVO Annual Meeting Abstract  |   April 2010
Conjunctival Changes Induced by Fixed Combinations of Prostaglandin Analogues and Timolol Maleate: An Immunohistochemical Study
Author Affiliations & Notes
  • H. H. Russ
    Ophthalmology, Instituto Graefe de Oftalmologia, Curitiba, Brazil
    Ophthalmology, Federal University of Sao Paulo, São Paulo, Brazil
  • N. V. Lima
    Ophthalmology, Federal University of Minas Gerais, Hospital São Geraldo, Brazil
  • F. Montiani-Ferreira
    Veterinary Ophthalmology, Federal University of Parana, Curitiba, Brazil
    Veterinary Ophthalmology, Michigan State University, East Lansing, Michigan
  • P. Rose
    Veterinary Ophthalmology, Federal University of Parana, Curitiba, Brazil
  • L. Noronha
    Pathology, University Catholic of Parana, Curitiba, Brazil
  • Footnotes
    Commercial Relationships  H.H. Russ, None; N.V. Lima, None; F. Montiani-Ferreira, None; P. Rose, None; L. Noronha, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 3160. doi:
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      H. H. Russ, N. V. Lima, F. Montiani-Ferreira, P. Rose, L. Noronha; Conjunctival Changes Induced by Fixed Combinations of Prostaglandin Analogues and Timolol Maleate: An Immunohistochemical Study. Invest. Ophthalmol. Vis. Sci. 2010;51(13):3160.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : To compare immunohistochemical changes induced by antiglaucoma fixed combination therapies.

Methods: : 30 New Zealand rabbits were divided in 3 groups of 10 animals.The left eyes were treated with one daily drop of timolol maleate 0.5% associated with bimatoprost 0.03% (Ganfort® - Allergan), travoprost 0.004% (Duo-Travatan® - Alcon) and latanoprost 0.005% (Xalacom® - PharmaciaPfizer) for 30 days. The right eyes served as controls. All rabbits were euthanized, enucleated and fixed with 10% formaldehyde for 24 hours. The material were processed for immunohistochemistry using anti-factor VIII and anti-actin antibodies and then evaluated by light microscopy. IHC images were analyzed by the Image Pro-Plus 4.5 software. Parameters evaluated were: number of reactive blood vessels to factor VIII and qualitative evaluation of fibroblasts labeled for actin.The scores for fibrosis were classified from minimum to severe. One-way ANOVA with significance level of 5% followed by Tukey-Kramer post hoc tests were used to analyze numerical variables (factor VIII). The evaluation of the ordinal categorical variables (actin-labelling) was performed by means of analysis descriptive statistics and Fischer Exact Test.

Results: : A significant increase in factor VIII-labeled blood vessels was observed in eyes treated with latanoprost+timolol (5.56 ± 1.6; P=0.004) and bimatoprost+timolol (4.5 ± 1.6; P=0.046).The increase was significant higher in eyes latanoprost+timolol compared to travaprost+timolol (P=0.018).Conversely in eyes treated with travoprost+timolol a trend towards a higher intensity of actin-labeling was seen , followed by bimatoprost+timolol and lastly by latanoprost+timolol.

Conclusions: : All fixed combinations of prostaglandin analogues and timolol caused a certain degree of conjunctival inflammatory response, either by provoking a degree of subendotelial of fibrosis or an increase in numbers of reactive blood vessels. Eyes treated with latanoprost+timolol showed a greater number of reactive blood vessels. The degree of fibrosis was more intense in eyes treated with the association of travoprost+timolol.

Keywords: conjunctiva • drug toxicity/drug effects • pathology: experimental 

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