April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Microplasmin Improves Surgical Outcome in a Rabbit Model for Trabeculectomy
Author Affiliations & Notes
  • I. Stalmans
    Ophthalmology, Universitaire Ziekenhuizen Leuven (UZLeuven), Leuven, Belgium
    Laboratory of Ophthalmology,
    Katholieke Universiteit Leuven (KULeuven), Leuven, Belgium
  • T. Van Bergen
    Laboratory of Ophthalmology,
    Katholieke Universiteit Leuven (KULeuven), Leuven, Belgium
  • S. Van de Veire
    Ophthalmology, Universitaire Ziekenhuizen Leuven (UZLeuven), Leuven, Belgium
    Laboratory of Ophthalmology,
    Katholieke Universiteit Leuven (KULeuven), Leuven, Belgium
  • L. Moons
    Department of Biology,
    Katholieke Universiteit Leuven (KULeuven), Leuven, Belgium
  • E. Vandewalle
    Ophthalmology, Universitaire Ziekenhuizen Leuven (UZLeuven), Leuven, Belgium
    Laboratory of Ophthalmology,
    Katholieke Universiteit Leuven (KULeuven), Leuven, Belgium
  • Footnotes
    Commercial Relationships  I. Stalmans, Thrombogenics, F; Thrombogenics, P; T. Van Bergen, Thrombogenics, F; S. Van de Veire, None; L. Moons, None; E. Vandewalle, None.
  • Footnotes
    Support  Baekeland grant from the IWT (Agency for Innovation through Science and Technology)
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 3200. doi:
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    • Get Citation

      I. Stalmans, T. Van Bergen, S. Van de Veire, L. Moons, E. Vandewalle; Microplasmin Improves Surgical Outcome in a Rabbit Model for Trabeculectomy. Invest. Ophthalmol. Vis. Sci. 2010;51(13):3200.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : This study was designed to study the efficacy and safety of Microplasmin as an anti-scarring agent in a rabbit model of trabeculectomy.

Methods: : The effect of Microplasmin was investigated in vivo in a rabbit model for glaucoma surgery. Clinical outcome measures were intra-ocular pressure, bleb area, and side effects on slit lamp examination. Moreover, (immuno-) histochemical analysis of the eyes was performed, with quantification of inflammation (CD 45) and collagen deposition (Trichrome and Sirius Red).In the first experiment (n=3), topical Microplasmin drops were compared to placebo drops. In the second experiment (n=5), subconjunctival Microplasmin injection was compared to placebo injection. In the third experiment (n=10), intracameral Microplasmin injection was compared to placebo injection. In the last experiment a combination of intracameral Microplasmin and topical drops was compared to placebo injection and drops. All experiments were conducted by a masked observer. The aquous solution of microplasmin used in all experiments was not optimized for use as drops or in subconjunctival injection.

Results: : Microplasmin combination therapy significantly augmented the bleb area over the 30 days of follow-up (p=0.05). There was a trend that the single anterior chamber injection augmented the bleb area in the first week compared to control (p= 0.08). In contrast the beneficial effects of microplasmin after topical administration alone or subconjunctival Microplasmin trabeculectomy were absent (p=0.73, 0.90 respectively). No significant changes in collagen deposition and inflammation in the anterior chamber or in the conjunctiva were noticed.

Conclusions: : The combination of anterior chamber injection and topical drops improved surgical outcome of trabeculectomy in a rabbit model.

Keywords: wound healing 
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