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A. Grise-Dulac, Jr., A. Denoyer, S. Bauer, E. Warcoin, A. Pauly, P. Hamard, W. Rostene, F. Brignole-Baudouin, C. Baudouin; Fractalkine and Cx3cr1 in Human Trabecular Cells and Trabecular Meshwork. Invest. Ophthalmol. Vis. Sci. 2010;51(13):3229.
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Primary open angle glaucoma (POAG) includes morphological and cellular changes in the trabecular meshwork (TM) inducing an elevation of intraocular pressure (IOP). Chemokines are small proteins involved in the leukocyte chemotaxis to pathological tissues during inflammation. The chemokine Fractalkine (CX3CL1) and its receptor CX3CR1 have been suggested to play an important role in the pathogenesis of several inflammatory or degenerative disorders. We hypothesized that CX3CL1/CX3CR1 interaction could be involved in the changes in the TM during POAG.The objective of this study was to investigate both CXCL1 and CX3CR1 in trabecular cells under normal and pathological conditions.
Expressions of fractalkine and its receptor were assessed by immunohistochemistry (IHC), flow cytometry (FCM) and RT-qPCR in two different human trabecular cell lines (glaucomatous and non-glaucomatous) under basal conditions and after stimulations with TNFα or Benzalkonium Chloride (BAC). Complementarily, IHC and RT-qPCR were performed on 22 human glaucomatous TM obtained during a non penetrating glaucoma surgery.
Fractalkine and CX3CR1 were detected in both human trabecular cell lines and in human glaucomatous TM. CX3CR1 expression was significantly higher in the glaucomatous cell line than in non-glaucomatous cell line, whereas fractalkine expression was not different. Stimulation with TNFα and with various concentrations of BAC induced an overexpression of both fractalkine and CX3CR1 in the trabecular cell lines.
We reported a basal expression of fractalkine and its receptor in human trabecular cell lines and in human glaucomatous TM. Moreover, the reported BAC-induced enhancement of fractalkine/CX3CR1 expressions suggests that chronic exposure to topical preservatives could play a role in the TM inflammation in glaucomatous treated eyes.
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