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S. Ishikawa, S. Okinami; Retinal Toxicity of Atelocollagen, a Compound of Extends siRNA Effect. Invest. Ophthalmol. Vis. Sci. 2010;51(13):3303.
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© ARVO (1962-2015); The Authors (2016-present)
Atelocollagen have an effect to extend small interfering RNA (siRNA) effect. In 2008 ARVO meeting, we reported caspase 3 and caspase 9 siRNA protect the retinal ganglion cell damage induced by transient ischemic injury in short term. Atelocollagen and siRNA complex is expected to extend siRNA effect. On this assumption, we discussed retinal toxicity of atelocollagen.
Seven week-old male Wistar rats were used. Five micro litter Atelocollagen was injected into vitreous cavity. Buffer solution was injected in eyes as control. Retinal ganglion cells were labeled with Fluoro-Gold retrogradely 3 day before injection. Two days after the injection, flat-mounts of the retina were examined by fluorescence microscopy and retinal segmentation samples were examined by optical microscopy.
The number of retinal ganglion cell was 1077.0 (+/-88.6) /mm2 in control group, the, and 961.7 (+/-62.2) /mm2 in Atelocollagen group. There was no significant difference in each group (p=0.14, Mann-Whitney U test). In the retinal thickness measurements, there were no significant differences in RGC+IPL, INL+OPL and ONL.
Atelocollagen was not shown retinal toxicity and it is possible to maintain siRNA effect in vitreous cavity.
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