April 2010
Volume 51, Issue 13
ARVO Annual Meeting Abstract  |   April 2010
Retinal Toxicity of Atelocollagen, a Compound of Extends siRNA Effect
Author Affiliations & Notes
  • S. Ishikawa
    Saga Univ Faculty of Medicine, Saga, Japan
  • S. Okinami
    Ophthalmology, Saga Univ Medical School, Saga, Japan
  • Footnotes
    Commercial Relationships  S. Ishikawa, None; S. Okinami, None.
  • Footnotes
    Support  Grant-in-Aid forScientific Research (C) by Japan Society for the Promotion of Science
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 3303. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      S. Ishikawa, S. Okinami; Retinal Toxicity of Atelocollagen, a Compound of Extends siRNA Effect. Invest. Ophthalmol. Vis. Sci. 2010;51(13):3303.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Purpose: : Atelocollagen have an effect to extend small interfering RNA (siRNA) effect. In 2008 ARVO meeting, we reported caspase 3 and caspase 9 siRNA protect the retinal ganglion cell damage induced by transient ischemic injury in short term. Atelocollagen and siRNA complex is expected to extend siRNA effect. On this assumption, we discussed retinal toxicity of atelocollagen.

Methods: : Seven week-old male Wistar rats were used. Five micro litter Atelocollagen was injected into vitreous cavity. Buffer solution was injected in eyes as control. Retinal ganglion cells were labeled with Fluoro-Gold retrogradely 3 day before injection. Two days after the injection, flat-mounts of the retina were examined by fluorescence microscopy and retinal segmentation samples were examined by optical microscopy.

Results: : The number of retinal ganglion cell was 1077.0 (+/-88.6) /mm2 in control group, the, and 961.7 (+/-62.2) /mm2 in Atelocollagen group. There was no significant difference in each group (p=0.14, Mann-Whitney U test). In the retinal thickness measurements, there were no significant differences in RGC+IPL, INL+OPL and ONL.

Conclusions: : Atelocollagen was not shown retinal toxicity and it is possible to maintain siRNA effect in vitreous cavity.

Keywords: neuroprotection • ganglion cells • injection 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.