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D. Borchman, G. N. Foulks, M. C. Yappert; Changes in Human Meibum With Age and Meibomian Gland Dysfunction. Invest. Ophthalmol. Vis. Sci. 2010;51(13):3383.
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Changes in the phase transition temperatures and conformation of human meibum lipid with age and meibomian gland dysfunction (MGD) have been quantified but with analysis of less than 1% of the infrared spectrum. The remaining 99 % of the spectra were further analyzed with principal component analysis (PCA) and confirm our previous studies as well as providing further insights into changes that occur in meibum with age, disease and therapy.
Infrared spectra of meibum from 41 patients diagnosed with MGD (Md) and 27 normal donors (Mn) were measured. Principal component analysis (PCA) was used to quantify the variance between the spectra.
Age related differences.
PCA is an excellent chemometric algorithm that may be used to diagnose and characterize MGD and age related changes in human meibum. With additional samples measured in the future, the training set will improve even more. PCA could be used by an untrained person as an initial diagnostic screening procedure for MGD. The eigenvectors that define the variations in the spectra with age and disease provide clues to the compositional and functional changes that occur in meibum with age and disease
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