April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Antibacterial, Antifungal and Antiamoebal Properties of a Novel Multi-Purpose Solution
Author Affiliations & Notes
  • R. N. Borazjani
    Research, Alcon Labs, Fort Worth, Texas
  • C. McAnally
    Research, Alcon Labs, Fort Worth, Texas
  • M. Callan
    Research, Alcon Labs, Fort Worth, Texas
  • L. McNamee
    Research, Alcon Labs, Fort Worth, Texas
  • R. Walters
    Research, Alcon Labs, Fort Worth, Texas
  • S. Neelam
    Research, Alcon Labs, Fort Worth, Texas
  • G. Kappell
    Research, Alcon Labs, Fort Worth, Texas
  • A. Allen
    Research, Alcon Labs, Fort Worth, Texas
  • R. Smith
    Research, Alcon Labs, Fort Worth, Texas
  • Footnotes
    Commercial Relationships  R.N. Borazjani, Alcon Labs, E; C. McAnally, Alcon Labs, E; M. Callan, Alcon Labs, E; L. McNamee, Alcon Labs, E; R. Walters, Alcon Labs, E; S. Neelam, Alcon Labs, E; G. Kappell, Alcon Labs, E; A. Allen, Alcon Labs, E; R. Smith, Alcon Labs, E.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 3414. doi:
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      R. N. Borazjani, C. McAnally, M. Callan, L. McNamee, R. Walters, S. Neelam, G. Kappell, A. Allen, R. Smith; Antibacterial, Antifungal and Antiamoebal Properties of a Novel Multi-Purpose Solution. Invest. Ophthalmol. Vis. Sci. 2010;51(13):3414.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To investigate the antimicrobial properties of a new contact lens care solution (CLS) with a dual disinfection system designed for silicone hydrogel contact lenses against Bacterial, Fungal, and Acanthamoeba spp.

Methods: : We evaluated efficacy of a new CLS against Acanthamoeba castellanii, Staphylococcus aureus, Pseudomonas aeruginosa, Serratia marcescens, Candida albicans, and Fusarium solani using batteries of both conventional and novel microbiological test methods. The test methods included time kill studies (Stand-alone), contact lens disinfection studies (Regimen), Preservative Efficacy Testing (PET), Antimicrobial Efficacy End Point (AEEMC), Encystment studies, and Zone of Inhibition studies (ZOI). Regimen, AEEMC, and ZOI were evaluated against Acuvue* OASYS, PureVision*, Air Optix Night and Day*, Biofinity*, Acuvue II* and Biomedics 38* contact lenses.

Results: : After six hours of disinfection time, the new CLS showed more than 1 log kill against cysts of A. castellanii and caused no Encystment (<1%) for the trophozoites of A. castellanii. Time kill studies showed more than 10 log "combined" reductions for all three bacteria and more than 5 log "combined" reductions for both fungi. The regimen results for bacterial, fungal and Acanthamoeba spp. showed <10 cfu/lens after soaking for six hours in this new CLS and application of the recommended cleaning steps for the CLS. The results of AEEMC showed no survivors after day 7 in the lens cases. The new CLS showed preservative efficacy against bacteria (> 3 log reduction for each bacteria) and Fungi (>1 log reduction for each fungi) up to 28 days after a re-challenge at day 14.

Conclusions: : We have demonstrated the antibacterial, antifungal and antiamoebal properties of a new CLS through batteries of microbiological methodologies. The dual disinfection system of PolyQuad® and Aldox® contributes to the antimicrobial efficacy of this new solution.

Keywords: Acanthamoeba • contact lens 
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