April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Immunohistochemical Profile Comparing Nodular and Morphea Type Basal Cell Carcinomas and Papillomas of the Eyelid
M. E. Orellana; G. A. Novais; D. M. Abdulmannan; S. C. Maloney; S. Di Cesare; M. N. Burnier, Jr.
Author Affiliations & Notes
  • M. E. Orellana
    Ocular Pathology Section, Universidad Central de Venezuela, Caracas, Venezuela
    Henry C Witelson Ocular Pathology Laboratory,
    McGill University, Montreal, Quebec, Canada
  • G. A. Novais
    Henry C Witelson Ocular Pathology Laboratory,
    McGill University, Montreal, Quebec, Canada
  • D. M. Abdulmannan
    Henry C Witelson Ocular Pathology Laboratory,
    McGill University, Montreal, Quebec, Canada
  • S. C. Maloney
    Henry C Witelson Ocular Pathology Laboratory,
    McGill University, Montreal, Quebec, Canada
  • S. Di Cesare
    Henry C Witelson Ocular Pathology Laboratory,
    McGill University, Montreal, Quebec, Canada
  • M. N. Burnier, Jr.
    Henry C Witelson Ocular Pathology Laboratory,
    Ophthalmology,
    McGill University, Montreal, Quebec, Canada
  • Footnotes
    Commercial Relationships  M.E. Orellana, None; G.A. Novais, None; D.M. Abdulmannan, None; S.C. Maloney, None; S. Di Cesare, None; M.N. Burnier, Jr., None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 3517. doi:
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      M. E. Orellana, G. A. Novais, D. M. Abdulmannan, S. C. Maloney, S. Di Cesare, M. N. Burnier, Jr.; Immunohistochemical Profile Comparing Nodular and Morphea Type Basal Cell Carcinomas and Papillomas of the Eyelid. Invest. Ophthalmol. Vis. Sci. 2010;51(13):3517.

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Abstract

Purpose: : Basal cell carcinoma (BCC) is the most common skin and periocular malignancy. Of all skin BCC, 86% involves the head; 14% to 16.6% is located in the periocular area. BCC can be divided into different subtypes, of which nodular type is the most common with a relatively good prognosis. On the other hand, morphea type places patients at high risk for more aggressive disease. The aim of this study was to determine immunohistochemical differences between BCC and non-invading lesions such as squamous cell papillomas (SCP); and also to compare the immunohistochemical behavior of two different subtypes of BCC (nodular and morphea).

Methods: : Twenty eight BCC (17 nodular, and 11 morphea), and ten SCP cases were histopathologically diagnosed and further analyzed by immunohistochemistry using the following antibodies: Epithelial Cell Adhesion Molecule (EpCAM), cytokeratin 8 (CAM 5.2), lysyl oxidase-like 2 (LOXL2), and Collagen IV. Epithelial and stromal (dermal) immunostaining were assessed in terms of intensity and extent for each marker.

Results: : Cytoplasmic expression of EpCAM and CAM 5.2 was strong in 24 of 28 (86%) and 27 of 28 (96%) BCC cases, respectively. Both markers were negative in all SCP cases (Fisher Exact Test, p < 0.0001). Peritumoral stromal tissue was positive for Collagen IV in 13 of 28 BCC cases, while was negative in all SCP cases (Fisher Exact Test, p < 0.0001). Immunostaining for LOXL2 was similar in both groups (60.71% BCC and 60% SCP). The comparison between solid and morphea BCC type showed no statistically significant differences for any of these markers.

Conclusions: : These results suggest that the cells forming BCC lesions are different clones from the squamous cell papilloma cells, and those cells produce focal collagen changes due to their invasiveness. The differences between the behavior of nodular and morphea BCC types in our study was not related to promoting epithelial-mesenchymal transition, tested with LOXL2.

Keywords: eyelid • immunohistochemistry • pathology techniques 
© 2010, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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