April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Characterization of an Immunohistochemical Panel for Diagnosis of Sebaceous Carcinoma of the Eyelid
Author Affiliations & Notes
  • A. N. Odashiro
    Ocular Pathology, Pathology,
    McGill University, Montreal, Quebec, Canada
    Federal University of Mato Grosso do Sul, Campo Grande, Brazil
  • P. R. P. Odashiro
    Ocular Pathology, Pathology,
    McGill University, Montreal, Quebec, Canada
    Ophthalmology, Federal University of Sao Paulo, Sao Paulo, Brazil
  • R. P. Gasparini
    Pathology, Saúde e Desenvolvimento do Centro-Oeste,
    Federal University of Mato Grosso do Sul, Campo Grande, Brazil
  • P. I. Fernandes
    Ophthalmology, Sociedade Beneficente de Campo Grande - Santa Casa, Campo Grande, Brazil
  • B. F. Fernandes
    Ocular Pathology, Pathology,
    McGill University, Montreal, Quebec, Canada
  • S. Di Cesare
    Ocular Pathology, Pathology,
    McGill University, Montreal, Quebec, Canada
  • S. C. Maloney
    Pathology, Saúde e Desenvolvimento do Centro-Oeste,
    McGill University, Montreal, Quebec, Canada
  • E. Antecka
    Ocular Pathology, Pathology,
    McGill University, Montreal, Quebec, Canada
  • C. Martins
    Ocular Pathology, Pathology,
    McGill University, Montreal, Quebec, Canada
  • M. N. Burnier, Jr.
    Ophthalmology,
    McGill University, Montreal, Quebec, Canada
  • Footnotes
    Commercial Relationships  A.N. Odashiro, None; P.R.P. Odashiro, None; R.P. Gasparini, None; P.I. Fernandes, None; B.F. Fernandes, None; S. Di Cesare, None; S.C. Maloney, None; E. Antecka, None; C. Martins, None; M.N. Burnier, Jr., None.
  • Footnotes
    Support  CNPQ, FUNDECT
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 3520. doi:
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      A. N. Odashiro, P. R. P. Odashiro, R. P. Gasparini, P. I. Fernandes, B. F. Fernandes, S. Di Cesare, S. C. Maloney, E. Antecka, C. Martins, M. N. Burnier, Jr.; Characterization of an Immunohistochemical Panel for Diagnosis of Sebaceous Carcinoma of the Eyelid. Invest. Ophthalmol. Vis. Sci. 2010;51(13):3520.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Sebaceous carcinoma (SC) is a tumor of the skin adnexae, more commonly located in the eyelid, and its diagnosis can be a challenge for the clinician and the ocular pathologist. Histopathologically, SC can mimic squamous cell carcinoma (SqCC) and basal cell carcinoma (BCC). Immunohistochemistry (IM) is a useful tool that could help ocular pathologists to make diagnosis of SC. Literature regarding IM and SC is scarce. The aim of this study is characterize a panel of IM antibodies for histopathological diagnosis of SC of the eyelid.

Methods: : 30 cases of eyelid tumors (10 SC, 10 BCC and 10 SqCC) were retrieved from the archives of the Ocular Pathology Department. A panel of IM antibodies was performed in all cases, including BRST-1, EMA, CAM 5.2, Ki67. A statistical analysis was performed.

Results: : All cases of SC were poorly differentiated, except two. 7 cases (70%) had features of SqCC and 3 (30%) of BCC. BRST-1 was positive in 100% of normal sebaceous glands and eccrine glands, when present. BRST-1 was diffusely positive in just 3 (30%) SC but was positive in all areas of sebaceous differentiation in 100% of the SC. BRST-1 was negative in all BCC and SqCC. EMA was positive in 100% of normal sebaceous glands and eccrine glands. EMA was diffusely positive in 60% of the SC (most with SqCC features) and in 90% of sebaceous differentiated areas of SC. It was positive in 70% of SqCC and negative in all cases of BCC. CAM5.2 was diffusely positive in 50% of SC and in 70% of sebaceous differentiated areas of SC. It was positive in 50% of BCC and 10% of SqCC. The proliferative index (ki67) had no differences between the tumors.

Conclusions: : BRST-1, EMA and CAM5.2 can be used as IM panel to make the correct diagnosis of SC. BRST-1 is the single most important antibody. BRST-1 plus EMA and BRST-1 plus CAM5.2 can help distinguish SC from BCC and SqCC, respectively. All 3 antibodies stain sebaceous areas and the location of staining of each antibody should be interpreted carefully.

Keywords: immunohistochemistry • tumors • eyelid 
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