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J.-M. A. Parel, E. Arrieta, M. C. Aguilar, E. Lee, F. Ponce, E. Hernandez, T. Albini, S. Dubovy, M. Beccaro, C. Mariotti; Asymmetric Perfluorocarbon Liquids (aPFCLs) and Combination aPFCLs/Silicone Oil (PDMS): Pilot in-vivo Biocompatibility Study. Invest. Ophthalmol. Vis. Sci. 2010;51(13):3605.
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To assess the stability and biocompatibility of aPFCLs, combination aPFCLs with PDMS, and control polymers as a vitreous substitute.
14 NZW rabbits were used for this ongoing blind study. 4 different polymers consisting of either an aPFCLs, or varying concentrations of PDMS combined with an aPFCLs (Al.Chi.Mi.A, SRL, Italy) and 4 control polymers: 1000 cts, 5000 cts PDMS (Al.Chi.Mi.A, SRL, Italy), F6H8 (semi-fluorinated alkane with C14H17F13- Fluoron GmbH) and DENSIRON 68 (mixture of F6H8 and PDMS 5000 cts) were randomly implanted. Lens phacoemulsification (PE) and total vitrectomy was performed under deep IM anesthesia: PE of lens contents and cornea was sutured, peritomy from 10 o'clock and 2 o'clock, 2 scleral mattress 7-0 Vicryl sutures (VS) were preplaced, fluorescein stain was injected into the vitreous cavity, a total vitrectomy was done with a 20G vitrectome (Storz, Bausch and Lomb, USA), injection of polymer ~1.8-1.0cc above the retina, VS were tightened, conjunctiva was closed. The rabbits were examined at POD 1 and 3 by slit-lamp and at POD 7, 14, 21, and 28 and then monthly under the operation microscope. Half the animals will be euthanized at POD 28 and half at POD 90. Histological analysis will be performed on all operated and control eyes.
There were no clinical signs of infection in all rabbits. At POD 28 all animals had a normal cornea and conjunctiva, except for those who received F6H8 or DENSIRON 68 which had chronic diffuse corneal edema. Lens material regrowth is seen throughout the periphery in all operated animals. PDMS controls: the retina remained normal even at POD 60. F6H8 control: the medullary ray (MR) appeared white at POD 14. DENSIRON 68 control: vessels of the MR appeared thin at POD 21. Both aPFCLs (C11H11 F13O) and (C9H11 F9O): the retina remained normal until POD 7, aPFCL (C9H11 F9O)/PDMS: retinal vessels appeared slightly tortuous at POD 14, aPFCL (C11H11 F13O)/PDMS: retinal vessels appeared normal at POD 21 (ongoing).
This ongoing study indicated the aPFCL (C11H11 F13O)/PDMS was best tolerated in the rabbit model and could potentially be effectively used in vitreoretinal surgery as a vitreous substitute.Support: Florida Lions Eye Bank; Abeamed Inc; NIH Center Grant P30EY14801; Research to Prevent Blindness; Henri and Flore Lesieur Foundation
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