April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Retinal Progenitor Apoptosis and Aberrant Photoreceptor Morphology Characterise the Dying on Edge (Dye) Zebrafish Mutant
L. Shine; B. Sapetto-Rebow; A. L. Reynolds; Y. Alvarez; S. McLoughlin; B. N. Kennedy
Author Affiliations & Notes
  • L. Shine
    Conway Institute, University College Dublin, Dublin, Ireland
  • B. Sapetto-Rebow
    Conway Institute, University College Dublin, Dublin, Ireland
  • A. L. Reynolds
    Conway Institute, University College Dublin, Dublin, Ireland
  • Y. Alvarez
    Conway Institute, University College Dublin, Dublin, Ireland
  • S. McLoughlin
    Conway Institute, University College Dublin, Dublin, Ireland
  • B. N. Kennedy
    Conway Institute, University College Dublin, Dublin, Ireland
  • Footnotes
    Commercial Relationships  L. Shine, None; B. Sapetto-Rebow, None; A.L. Reynolds, None; Y. Alvarez, None; S. McLoughlin, None; B.N. Kennedy, None.
  • Footnotes
    Support  Science Foundation Ireland Award 08/RFP/GEN1126 (BNK)
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 3655. doi:
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      L. Shine, B. Sapetto-Rebow, A. L. Reynolds, Y. Alvarez, S. McLoughlin, B. N. Kennedy; Retinal Progenitor Apoptosis and Aberrant Photoreceptor Morphology Characterise the Dying on Edge (Dye) Zebrafish Mutant. Invest. Ophthalmol. Vis. Sci. 2010;51(13):3655.

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Abstract

Purpose: : To characterise the phenotypes that affect visual function in the dye zebrafish mutant.

Methods: : Optokinetic response (OKR) screens of ENU-mutagenised zebrafish identified a family with a recessive mutation disrupting visual function. Retinal morphology was characterised by light microscopy and electron microscopy. Cellular lamination and retinal cell type markers were characterised by immunohistochemistry using zpr1, 4C12, 5E11, PKC and Cralbp antibodies. Cell death levels were determined by TUNEL staining. Hyaloid and trunk vasculature was analysed by alkaline phosphatase staining and circadian locomotion behaviour was investigated using Zebralab (Viewpoint Zebrafish Tracking System).

Results: : dye

Conclusions: : We have identified and partially characterised a novel zebrafish mutant with a visual function defect. Preliminary results suggest that this defect is caused by malformation of the RPE, abnormal photoreceptor morphology and progenitor apoptosis.

Keywords: retinal development • apoptosis/cell death • gene mapping 
© 2010, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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