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L. Shine, B. Sapetto-Rebow, A. L. Reynolds, Y. Alvarez, S. McLoughlin, B. N. Kennedy; Retinal Progenitor Apoptosis and Aberrant Photoreceptor Morphology Characterise the Dying on Edge (Dye) Zebrafish Mutant. Invest. Ophthalmol. Vis. Sci. 2010;51(13):3655.
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© ARVO (1962-2015); The Authors (2016-present)
To characterise the phenotypes that affect visual function in the dye zebrafish mutant.
Optokinetic response (OKR) screens of ENU-mutagenised zebrafish identified a family with a recessive mutation disrupting visual function. Retinal morphology was characterised by light microscopy and electron microscopy. Cellular lamination and retinal cell type markers were characterised by immunohistochemistry using zpr1, 4C12, 5E11, PKC and Cralbp antibodies. Cell death levels were determined by TUNEL staining. Hyaloid and trunk vasculature was analysed by alkaline phosphatase staining and circadian locomotion behaviour was investigated using Zebralab (Viewpoint Zebrafish Tracking System).
We have identified and partially characterised a novel zebrafish mutant with a visual function defect. Preliminary results suggest that this defect is caused by malformation of the RPE, abnormal photoreceptor morphology and progenitor apoptosis.
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