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M. Ugarte, L. Bentley, R. D. Cox, P. N. Bishop; Novel Murine Models for Proliferative Diabetic Retinopathy. Invest. Ophthalmol. Vis. Sci. 2010;51(13):3658.
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To evaluate novel murine models of diabetes and determine whether they develop diabetic retinopathy.
The MRC Harwell Mammalian Genetics Unit has developed a number of mouse models of diabetes by N-ethyl N-nitrosurea (ENU) mutagenesis, including two called SLUMP and SWEET P, These mice are on a C3H background, which is homozygous for a mutation in the cGMP phosphodiesterase gene, and they develop an early retinal degeneration. The mice were kept for up to one year prior to evaluation. To visualize the retinal vasculature eye cups and retinal flat mounts were stained with biotinylated isolectin B4 and a secondary antibody labelled with Alexa-488.
Control (non diabetic) C3H mice had an abnormal retinal vasculature with low vessel density. Over and above this the SLUMP and SWEETP mice demonstrated retinal vessel tortuosity, microglial activation and between 9-12 months developed preretinal neovascularisation.
The SLUMP and SWEETP mice demonstrate features of diabetic retinopathy including proliferative disease.
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