April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Novel Murine Models for Proliferative Diabetic Retinopathy
Author Affiliations & Notes
  • M. Ugarte
    School of Biomedicine, Faculty of Medical and Human Sciences, University of Manchester, Manchester M13 9PT, United Kingdom
  • L. Bentley
    Harwell Science and Innovation Campus, MRC Harwell Mammalian Genetics Unit, Oxfordshire OX11 0RD, United Kingdom
  • R. D. Cox
    Harwell Science and Innovation Campus, MRC Harwell Mammalian Genetics Unit, Oxfordshire OX11 0RD, United Kingdom
  • P. N. Bishop
    School of Biomedicine, Faculty of Medical and Human Sciences, University of Manchester, Manchester M13 9PT, United Kingdom
  • Footnotes
    Commercial Relationships  M. Ugarte, None; L. Bentley, None; R.D. Cox, None; P.N. Bishop, None.
  • Footnotes
    Support  Support for the NIHR Manchester Biomedical Research Centre
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 3658. doi:
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      M. Ugarte, L. Bentley, R. D. Cox, P. N. Bishop; Novel Murine Models for Proliferative Diabetic Retinopathy. Invest. Ophthalmol. Vis. Sci. 2010;51(13):3658.

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Abstract

Purpose: : To evaluate novel murine models of diabetes and determine whether they develop diabetic retinopathy.

Methods: : The MRC Harwell Mammalian Genetics Unit has developed a number of mouse models of diabetes by N-ethyl N-nitrosurea (ENU) mutagenesis, including two called SLUMP and SWEET P, These mice are on a C3H background, which is homozygous for a mutation in the cGMP phosphodiesterase gene, and they develop an early retinal degeneration. The mice were kept for up to one year prior to evaluation. To visualize the retinal vasculature eye cups and retinal flat mounts were stained with biotinylated isolectin B4 and a secondary antibody labelled with Alexa-488.

Results: : Control (non diabetic) C3H mice had an abnormal retinal vasculature with low vessel density. Over and above this the SLUMP and SWEETP mice demonstrated retinal vessel tortuosity, microglial activation and between 9-12 months developed preretinal neovascularisation.

Conclusions: : The SLUMP and SWEETP mice demonstrate features of diabetic retinopathy including proliferative disease.

Keywords: retinal neovascularization • diabetic retinopathy • pathobiology 
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