April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Neuroprotective Effect of an Anti-Oxidant, Lutein, on Light-Induced Retinal Degeneration
Author Affiliations & Notes
  • M. Sasaki
    Laboratory of Retinal Cell Biology, Keio University School of Medicine, Tokyo, Japan
    Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  • Y. Ozawa
    Laboratory of Retinal Cell Biology, Keio University School of Medicine, Tokyo, Japan
    Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  • T. Kurihara
    Laboratory of Retinal Cell Biology, Keio University School of Medicine, Tokyo, Japan
    Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  • S. Kubota
    Laboratory of Retinal Cell Biology, Keio University School of Medicine, Tokyo, Japan
    Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  • K. Yuki
    Laboratory of Retinal Cell Biology, Keio University School of Medicine, Tokyo, Japan
    Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  • K. Noda
    Laboratory of Retinal Cell Biology, Keio University School of Medicine, Tokyo, Japan
    Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Hokkaido, Japan
  • S. Kobayashi
    Wakasa Seikatsu Co., Kyoto, Japan
  • S. Ishida
    Laboratory of Retinal Cell Biology, Keio University School of Medicine, Tokyo, Japan
    Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Hokkaido, Japan
  • K. Tubota
    Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  • Footnotes
    Commercial Relationships  M. Sasaki, Wakasa Seikatsu Co., F; Y. Ozawa, Wakasa Seikatsu Co., F; T. Kurihara, None; S. Kubota, None; K. Yuki, None; K. Noda, None; S. Kobayashi, Wakasa Seikatsu Co., E; S. Ishida, None; K. Tubota, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 3690. doi:
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      M. Sasaki, Y. Ozawa, T. Kurihara, S. Kubota, K. Yuki, K. Noda, S. Kobayashi, S. Ishida, K. Tubota; Neuroprotective Effect of an Anti-Oxidant, Lutein, on Light-Induced Retinal Degeneration. Invest. Ophthalmol. Vis. Sci. 2010;51(13):3690.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To investigate the neuroprotective effect of lutein on retinal neural damage caused by photo-oxidative stress.

Methods: : Mice were fed with laboratory chow with or without 0.1% lutein and exposed to 5,000 lux fluorescent light for 3 hours. Retinal damage was estimated by analyzing (1) number of TUNEL positive cells, (2) outer nuclear layer (ONL) thickness, and (3) retinal function was measured by electroretinography (ERG).

Results: : Light exposure induced increase in TUNEL positive cells in ONL, leading to the reduction of ONL thickness and a-wave amplitude in ERG, as previously reported. (1) However, lutein diet decreased the light-induced apoptosis in ONL by 43.7% of the control (p<0.01). (2) Consistently, Lutein diet significantly avoided the reduction of ONL thickness (p<0.05). (3) Furthermore, mice fed with lutein diet showed higher level of a-wave amplitude in ERG than those fed with control chow after light exposure (p<0.01).

Conclusions: : Lutein contributed to preservation of better visual function by protecting photoreceptor cells from apoptosis after light exposure. Lutein has a neuroprotective effect in light-induced retinal degeneration.

Keywords: photoreceptors • antioxidants • apoptosis/cell death 
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