April 2010
Volume 51, Issue 13
ARVO Annual Meeting Abstract  |   April 2010
Iron and Manganese Corroles Are Neuroprotective for Serum-Deprived Retinal Neurons
Author Affiliations & Notes
  • M.-M. Catrinescu
    Ophthalmology, Hospital Maisonnueve Rosemont Research, Montreal University, Quebec, Canada
  • A. Kanamori
    Ophthalmology, Montreal University, Montreal University, Quebec, Canada
  • R. Beaubien
    Centre de Recherches HMR, Ophtalmologie, Montreal University, Quebec, Canada
  • W. Chan
    Ophthalmology, Hospital Maisonnueve Rosemont Research, Montreal University, Quebec, Canada
  • Z. Gross
    Schulich Faculty of Chemistry, Technion, Israel Institute of Technology, Israel Institute of Technology, Israel
  • L. A. Levin
    Ophthalmology, Univ Montreal/Univ Wisconsin, Montreal University, Quebec, Canada
  • Footnotes
    Commercial Relationships  M.-M. Catrinescu, None; A. Kanamori, None; R. Beaubien, None; W. Chan, None; Z. Gross, None; L.A. Levin, None.
  • Footnotes
    Support  Canadian Institutes for Health Research, Canadian Foundation for Innovation, Canadian Research Chairs program, Fonds de recherche en ophtalmologie de l’Université de Montréal
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 3712. doi:
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      M.-M. Catrinescu, A. Kanamori, R. Beaubien, W. Chan, Z. Gross, L. A. Levin; Iron and Manganese Corroles Are Neuroprotective for Serum-Deprived Retinal Neurons. Invest. Ophthalmol. Vis. Sci. 2010;51(13):3712.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : Corroles are tetrapyrrolic macrocycles that have come under increased attention because of their unique capabilities for oxidation catalysis, reduction catalysis, and biomedical applications. Corrole-metal complexes (metallocorroles) can decompose a variety of reactive oxygen species (ROS), often more efficiently than analogous metalloporphyrins. We investigated whether bipolar Fe-, Mn- and Ga-corroles have neuroprotective effects on neurons and reduce ROS in vitro and in vivo.

Methods: : Undifferentiated and differentiated RGC-5 neuronal precursor cells were induced to undergo apoptosis by serum deprivation for 48 hours. Differentiation of RGC-5 cells was induced with 316 nM staurosporine. The level of cell death with or without these metallocorroles was monitored by XTT assay and by calcein-AM/propidium iodide assay. RGC-5 ROS were measured with hydroethidine (HEt), a superoxide indicator. In vivo, real-time imaging using a confocal scanning laser ophthalmoscope (CSLO) identified the production of ROS within individual rat RGCs after optic nerve transection. Intraocular ROS was visualized by intravitreal administration of HEt.

Results: : RGC-5 cell death after serum deprivation was significantly decreased by Fe- and Mn-corroles, but not Ga-corrole. This correlated with the ability of Fe- and Mn-corroles but not Ga-corrole to dismutate intracellular superoxide, measured by fluorescence after HEt administration. In vivo, intravitreal Fe- and Mn-corroles, but not Ga-corrole decreased retinal HEt-positivity after optic nerve transection.

Conclusions: : Fe- and Mn-corroles could be candidate drugs for delaying RGC death after axonal injury in optic neuropathies via suppression of specific ROS.

Keywords: neuroprotection • apoptosis/cell death • antioxidants 

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