April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Visualization of the Anterior Laminar Cribrosa Surface (ALCS) in Normal and Experimental Glaucoma (EG) Eyes Using Spectral Domain Optical Coherence Tomography (SD-OCT)
Author Affiliations & Notes
  • N. G. Strouthidis
    Optic Nerve Head Research Laboratory,
    Devers Eye Institute, Portland, Oregon
  • H. Yang
    Optic Nerve Head Research Laboratory,
    Devers Eye Institute, Portland, Oregon
  • B. Fortune
    Discoveries in Sight,
    Devers Eye Institute, Portland, Oregon
  • I. A. Sigal
    Ocular Biomechanics Laboratory,
    Devers Eye Institute, Portland, Oregon
  • C. F. Burgoyne
    Optic Nerve Head Research Laboratory,
    Devers Eye Institute, Portland, Oregon
  • Footnotes
    Commercial Relationships  N.G. Strouthidis, Heidelberg Engineering, F; H. Yang, None; B. Fortune, None; I.A. Sigal, None; C.F. Burgoyne, Heidelberg Engineering, F.
  • Footnotes
    Support  R01-EY11610
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 3857. doi:
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      N. G. Strouthidis, H. Yang, B. Fortune, I. A. Sigal, C. F. Burgoyne; Visualization of the Anterior Laminar Cribrosa Surface (ALCS) in Normal and Experimental Glaucoma (EG) Eyes Using Spectral Domain Optical Coherence Tomography (SD-OCT). Invest. Ophthalmol. Vis. Sci. 2010;51(13):3857.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose:
 

To characterize the extent and reproducibility of SD-OCT ALCS visualization within both eyes of 9 longitudinally imaged rhesus macaques with unilateral laser induced EG.

 
Methods:
 

9 rhesus macaques underwent baseline and post-laser optic nerve head (ONH) imaging (Heidelberg Spectralis; 870 nm center wavelength; 15º, 80 radial B-scan pattern) in both eyes under isoflurane anesthesia with IOP manometrically stabilized at 10 mmHg. Two baseline (pre-laser) and two follow-up (post-laser) volumes were analyzed. The first follow-up was at the onset of HRT-detected surface height depression and the second was the most recent, or pre-sacrifice, acquisition. Using custom software, four masked operators delineated the ALCS and the neural canal opening (NCO) in alternate B-scans (40/volume). To identify the extent and reproducibility of delineated ALCS within each ONH, each delineated ALCS point was projected to the NCO reference plane. NCO area was divided into 100 equal sized regions and the number of regions with two or more ALCS points in all 4 volumes was assessed.

 
Results:
 

‘Shared’ ALCS delineation points (present in all four volumes) for both eyes of all 9 animals at the second follow-up are shown in the figure. While the extent of ‘missing’ areas is variable between animals, in most eyes they directly correspond to the location of the central retinal vasculature. No systematic effect of time was found for either the percentage of LC marks retained (p = 0.52, repeated measures ANOVA) or the percentage area retained (p = 0.65), suggesting that the area of ALCS marked was reliable over time in both control and EG eyes. The average area marked was 66.6% in control eyes and 66.2% in EG eyes.

 
Conclusions:
 

Relatively large areas of the ALCS were detected and reproducibly delineated in longitudinal SD-OCT (870 nm) images of the ONH in both control and glaucomatous eyes. A formal comparison of ALCS visualization using 870 and 1060 nm center wavelength SD-OCT imaging will commence shortly.  

 
Keywords: optic disc • imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) 
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