April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Exacerbation of Corneal Scarring in HSV-1 gK Immunized Mice Correlates With Elevation of CD8+CD25+ T Cells in Corneas of Ocularly Infected Mice
Author Affiliations & Notes
  • S. J. Allen
    Ophthalmology,
    Cedars-Sinai Medical Center, Los Angeles, California
  • K. R. Mott
    Ophthalmology,
    Cedars-Sinai Medical Center, Los Angeles, California
  • A. V. Ljubimov
    Ophthalmology Research Laboratories,
    Cedars-Sinai Medical Center, Los Angeles, California
  • H. Ghiasi
    Ophthalmology Research,
    Cedars-Sinai Medical Center, Los Angeles, California
  • Footnotes
    Commercial Relationships  S.J. Allen, None; K.R. Mott, None; A.V. Ljubimov, None; H. Ghiasi, None.
  • Footnotes
    Support  This work was supported by Public Health Service grant EY13615 from the National Eye Institute to HG.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 3868. doi:
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      S. J. Allen, K. R. Mott, A. V. Ljubimov, H. Ghiasi; Exacerbation of Corneal Scarring in HSV-1 gK Immunized Mice Correlates With Elevation of CD8+CD25+ T Cells in Corneas of Ocularly Infected Mice. Invest. Ophthalmol. Vis. Sci. 2010;51(13):3868.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : We have shown previously that exacerbation of corneal scarring (CS) in HSV-1 glycoprotein K (gK) immunized mice was associated with CD8+ T cells. In this study, we investigated the type and the nature of the CD8+ T cell responses that are involved in the exacerbation of gK-induced CS.

Methods: : BALB/c mice were vaccinated with baculovirus expressed gK. Positive control mice were similarly immunized with baculovirus expressed HSV-1 gD and negative control mice were mock-immunized with wild-type baculovirus infected Sf9 cells. Twenty-one days after the third immunization, mice were ocularly infected with 2X105 PFU/eye of virulent HSV-1 strain McKrae. Infiltration into the cornea of CD4+, CD8+, CD25+, CD4+CD25+, CD8+CD25+, CD19+, CD40+, CD40L+, CD62L+, CD95+, B7-1+, B7-2+, MHC-I+, and MHC-II+ cells was monitored by immunohistochemistry on days 5, 10, 14, and 28 post infection (PI). The immunohistochemistry results were confirmed with TaqMan quantitative RT-PCR (qRT-PCR) and FACS.

Results: : On day 5 PI, we observed significantly higher numbers of CD8+CD25+ T cells in corneas of gK-immunized mice than that of gD- or mock-immunized mice by immunohistochemistry. qRT-PCR results confirmed the elevation of CD8 and CD25 transcripts in corneas of gK immunized mice. However, by day 28 PI only CD8+CD25- T cells were detected in the corneas of gK group. In addition, elevation of CD40, CD40L, B7-2, CD62L, active Caspase-3 and MHC-I was correlated with CS.

Conclusions: : This study demonstrates for the first time that the presence of CD8+CD25+ Treg in the cornea correlates with exacerbation of CS in the gK group.1

Keywords: herpes simplex virus • immunohistochemistry • keratitis 
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