April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Hydrogen Peroxide and Myeloperoxidase Released From Activated Neutrophils(pmn) Inhibit HSV-1 Growth
Author Affiliations & Notes
  • K. Hayashi
    Immunology/Virology,Lab.Immunol., National Eye Inst/NIH, Bethesda, Maryland
  • L. C. Hooper
    Immunology/Virology,Lab.Immunol., National Eye Inst/NIH, Bethesda, Maryland
  • B. Detrick
    Department of Pathology, Johns Hopkins University, BAltimore, Maryland
  • J. J. Hooks
    Immunology/Virology,Lab.Immunol., National Eye Inst/NIH, Bethesda, Maryland
  • Footnotes
    Commercial Relationships  K. Hayashi, None; L.C. Hooper, None; B. Detrick, None; J.J. Hooks, None.
  • Footnotes
    Support  This research was supported by the Intramural Research program of the NIH National Eye Institute.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 3875. doi:https://doi.org/
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      K. Hayashi, L. C. Hooper, B. Detrick, J. J. Hooks; Hydrogen Peroxide and Myeloperoxidase Released From Activated Neutrophils(pmn) Inhibit HSV-1 Growth. Invest. Ophthalmol. Vis. Sci. 2010;51(13):3875. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Neutrophils (PMN) are prominent infiltrating cells in acute herpetic keratitis as well as in chronic stromal keratitis. We studied virus (HSV-1) inhibitory activity by these chemoattacted PMN.

Methods: : Human peripheral blood neutrophils(CD16+)(PMN)were activated with supernatants obtained from corneal epithelial cells (HCE) and/or macrophages (THP-1 cells) infected with HSV or treated with HSV components(HSV DNA and HSV-Immune complex).Supernatants of PMN mixed with the HCE or THP-1 supernatants and supernatants of PMN+ treated HCE or THP-1 cells or cell associated products were assayed for hydrogen peroxide, myeloperoxidase and antiviral activities.

Results: : HSV did not grow in activated PMN cultures. When PMN were layered on top of the HSV infected Vero cell monolayers (,Vero Cell: PMN=1:100), virus growth was suppressed. Hydrogen peroxide and myeloperoxidase were detected in the supernatants of activated PMN. When hydrogen peroxide or myeloperoxidase was mixed with HSV and incubated 1 hour at 370C, plaque number of HSV was reduced 80-100% with the released concentration of hydrogen peroxide (800ng/ml-3,000ng/ml) and myeloperoxidase (3.5ng/ml-25ng/ml).

Conclusions: : The production of hydrogen peroxide and myeloperoxidase by activated PMN are potent inhibitor of HSV replication.

Keywords: herpes simplex virus • inflammation • keratitis 
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