Abstract
Purpose: :
Neutrophils (PMN) are prominent infiltrating cells in acute herpetic keratitis as well as in chronic stromal keratitis. We studied virus (HSV-1) inhibitory activity by these chemoattacted PMN.
Methods: :
Human peripheral blood neutrophils(CD16+)(PMN)were activated with supernatants obtained from corneal epithelial cells (HCE) and/or macrophages (THP-1 cells) infected with HSV or treated with HSV components(HSV DNA and HSV-Immune complex).Supernatants of PMN mixed with the HCE or THP-1 supernatants and supernatants of PMN+ treated HCE or THP-1 cells or cell associated products were assayed for hydrogen peroxide, myeloperoxidase and antiviral activities.
Results: :
HSV did not grow in activated PMN cultures. When PMN were layered on top of the HSV infected Vero cell monolayers (,Vero Cell: PMN=1:100), virus growth was suppressed. Hydrogen peroxide and myeloperoxidase were detected in the supernatants of activated PMN. When hydrogen peroxide or myeloperoxidase was mixed with HSV and incubated 1 hour at 370C, plaque number of HSV was reduced 80-100% with the released concentration of hydrogen peroxide (800ng/ml-3,000ng/ml) and myeloperoxidase (3.5ng/ml-25ng/ml).
Conclusions: :
The production of hydrogen peroxide and myeloperoxidase by activated PMN are potent inhibitor of HSV replication.
Keywords: herpes simplex virus • inflammation • keratitis