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K. Hayashi, L. C. Hooper, B. Detrick, J. J. Hooks; Hydrogen Peroxide and Myeloperoxidase Released From Activated Neutrophils(pmn) Inhibit HSV-1 Growth. Invest. Ophthalmol. Vis. Sci. 2010;51(13):3875.
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Neutrophils (PMN) are prominent infiltrating cells in acute herpetic keratitis as well as in chronic stromal keratitis. We studied virus (HSV-1) inhibitory activity by these chemoattacted PMN.
Human peripheral blood neutrophils(CD16+)(PMN)were activated with supernatants obtained from corneal epithelial cells (HCE) and/or macrophages (THP-1 cells) infected with HSV or treated with HSV components(HSV DNA and HSV-Immune complex).Supernatants of PMN mixed with the HCE or THP-1 supernatants and supernatants of PMN+ treated HCE or THP-1 cells or cell associated products were assayed for hydrogen peroxide, myeloperoxidase and antiviral activities.
HSV did not grow in activated PMN cultures. When PMN were layered on top of the HSV infected Vero cell monolayers (,Vero Cell: PMN=1:100), virus growth was suppressed. Hydrogen peroxide and myeloperoxidase were detected in the supernatants of activated PMN. When hydrogen peroxide or myeloperoxidase was mixed with HSV and incubated 1 hour at 370C, plaque number of HSV was reduced 80-100% with the released concentration of hydrogen peroxide (800ng/ml-3,000ng/ml) and myeloperoxidase (3.5ng/ml-25ng/ml).
The production of hydrogen peroxide and myeloperoxidase by activated PMN are potent inhibitor of HSV replication.
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