April 2010
Volume 51, Issue 13
ARVO Annual Meeting Abstract  |   April 2010
Antiadenovirus Activities of the Hcap-18, Antimicrobial Peptide, in vitro in Serotypes Inducing Keratoconjunctivitis
Author Affiliations & Notes
  • T. Tsukahara
    Opthalmology, Fukuoka University, Fukuoka-city, Japan
  • H. Inoue
    Opthalmology, Fukuoka University, Fukuoka-City, Japan
  • J. Huang
    Opthalmology, Fukuoka University, Fukuoka-City, Japan
  • H. Ozaki
    Opthalmology, Fukuoka University, Fukuoka-City, Japan
  • K. Kadonosono
    Opthalmology, Yokohama City University Medical Center, Yokohama-City, Japan
  • H. Ishiko
    Central Laboratories, Mitsubishi Chemical Medience, Inc., Tokyo, Japan
  • E. Uchio
    Opthalmology, Fukuoka University, Fukuoka-City, Japan
  • Footnotes
    Commercial Relationships  T. Tsukahara, None; H. Inoue, None; J. Huang, None; H. Ozaki, None; K. Kadonosono, None; H. Ishiko, None; E. Uchio, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 3879. doi:
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      T. Tsukahara, H. Inoue, J. Huang, H. Ozaki, K. Kadonosono, H. Ishiko, E. Uchio; Antiadenovirus Activities of the Hcap-18, Antimicrobial Peptide, in vitro in Serotypes Inducing Keratoconjunctivitis. Invest. Ophthalmol. Vis. Sci. 2010;51(13):3879.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : Many human adenovirus (HAdV) serotypes cause the most common external ocular viral infections. In particular, adenoviral conjunctivitis is known to be the major cause of acute infections associated with community and nosocomial epidemics. In innate immunity, antimicrobial peptides has an important role of self protection of ocular surface. hCAP (human cationic antimicrobial protein)-18 is a linear, α-helical peptides and consists of a conserved prosequence called the cathelin like domain and a C- terminal peptide named LL-37 . We investigated antiadenovirus activity of hCAP-18/LL-37 in vitro in several serotypes inducing keratoconjunctivitis.

Methods: : A549 cells were used for viral cell culture, and adenovirus serotypes 3, 4, 8, 19 and 37 were used. After calculating CC50 (50% cytotoxic concentration) of NCT by MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) method, HAdV was cultured with hCAP-18 for seven days, and extracted adenoviral DNA was quantitatively measured by real-time PCR.

Results: : EC50 (50% effective concentration) obtained by real-time PCR of hCAP-18 ranged between 118 and 270 µM. hCAP-18 showed an inhibitory effect against adenoviral proliferation in serotypes belonging to species B and D in a dose dependent manner. However, it was eneffective against type 4. hCAP-18/LL-37 was more effective for HAdV D species than B species.

Conclusions: : hCAP-18/LL-37 has inhibitory activity against HAdV types 3, 8, 19, 37 which induce keratoconjunctivitis. Our results indicate that hCAP-18/LL-37 may be possible candidate as eye drop for HAdV keratoconjunctivitis

Keywords: antiviral drugs • adenovirus • conjunctivitis 

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