April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Ex-vivo Model of Coxsackievirus Conjunctivitis Using Multilayered Human Conjunctival Epithelial Cells
J. Lee; E. Jun; J. Sunwoo; H. Lee; E. Kim; J.-H. Kim; J. Kim; M. Kim; Y. Kim; H. Tchah
Author Affiliations & Notes
  • J. Lee
    Department of Ophthalmology,
    University of Ulsan College of Medicine, Asan Medical Center, Seoul, Republic of Korea
  • E. Jun
    Department of Microbiology,
    University of Ulsan College of Medicine, Asan Medical Center, Seoul, Republic of Korea
    Research Institute for Biomacromolecules, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Republic of Korea
  • J. Sunwoo
    Department of Ophthalmology,
    University of Ulsan College of Medicine, Asan Medical Center, Seoul, Republic of Korea
  • H. Lee
    Department of Microbiology,
    University of Ulsan College of Medicine, Asan Medical Center, Seoul, Republic of Korea
    Research Institute for Biomacromolecules, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Republic of Korea
  • E. Kim
    Department of Ophthalmology,
    University of Ulsan College of Medicine, Asan Medical Center, Seoul, Republic of Korea
  • J.-H. Kim
    Department of Ophthalmology,
    University of Ulsan College of Medicine, Asan Medical Center, Seoul, Republic of Korea
  • J. Kim
    Department of Ophthalmology,
    University of Ulsan College of Medicine, Asan Medical Center, Seoul, Republic of Korea
  • M. Kim
    Department of Ophthalmology,
    University of Ulsan College of Medicine, Asan Medical Center, Seoul, Republic of Korea
  • Y. Kim
    Department of Microbiology,
    University of Ulsan College of Medicine, Asan Medical Center, Seoul, Republic of Korea
    Research Institute for Biomacromolecules, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Republic of Korea
  • H. Tchah
    Department of Ophthalmology,
    University of Ulsan College of Medicine, Asan Medical Center, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships  J. Lee, None; E. Jun, None; J. Sunwoo, None; H. Lee, None; E. Kim, None; J.-H. Kim, None; J. Kim, None; M. Kim, None; Y. Kim, None; H. Tchah, None.
  • Footnotes
    Support  Ministry of Health & Welfare of Republic of Korea Grant A08-0593
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 3882. doi:
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      J. Lee, E. Jun, J. Sunwoo, H. Lee, E. Kim, J.-H. Kim, J. Kim, M. Kim, Y. Kim, H. Tchah; Ex-vivo Model of Coxsackievirus Conjunctivitis Using Multilayered Human Conjunctival Epithelial Cells. Invest. Ophthalmol. Vis. Sci. 2010;51(13):3882.

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Abstract

Purpose: : To establish the ex-vivo model of coxsackievirus conjunctivitis.

Methods: : Human conjunctival epithelial cells were cultured for two weeks in serum free air-liquid interface system. Expression of CK 19 and MUC5AC in the cultured cells were studied by immunohistochemistry. Coxsackievirus A24 (CA24) was challenged for two hours in the cultured epithelial cells. Virus replication was measured in the cultured cells and media after 24 and 72 hours by TCID50 method. Histological changes were studied by H&E, DAPI and TUNEL staining.

Results: : Cultured human conjunctival epithelial cells formed multiple layers. Cultured cells showed positive immunohistochemical staining for CK19 and MUC5AC. Viral replication measured by TCID50 showed 10-fold increase at 72 hours compared to 24 hours. Mild cytopathic effect was found at 24 hours after viral inoculation. Cell damages with apoptotic features were remarkable at 72 hours.

Conclusions: : Multilayered cells showed characteristics of conjunctival epithelial cells. Virus replicated in the cultured cells inducing cytopathic effects. This conjunctivitis model would be useful in the future study to investigate pathogenesis of acute hemorrhagic conjunctivitis and the effectiveness of antiviral treatment.

Keywords: conjunctivitis • conjunctiva 
© 2010, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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