Abstract
Purpose: :
To evaluate bacterial eradication using UVA + Riboflavin used in Collagen Cross-Linking.
Methods: :
S. epidermidis
Results: :
Almost complete eradication( approx. 83 %) was achived using B2 (100 to 500 uM) and UVA for 60 minutes(10.8 J/m2). At higher concentrations of B2,1000 µM, 2000 µM and 2430 µM, the eradication was only around 40-60%. The thickness of the reaction-fluid could influence the UVA-exposure time for eradication as well as high B2-concentrations.A thinner layer was difficult to maintain as a complete surface over the entire area for UVA-exposure. Controls were significantly different. Increased UVA-dose was more efficient then higher B2-konc.
Conclusions: :
The UVA/B2 combination used in Collagen Cross-Linking can eradicate bacteria in fluid solutions. The combined effect was more efficient than UVA alone. The variations of B2- concentration seems to be of importance for the degree of bacterial reduction and a rather low dose seemed to be more efficient than a higher one. Excess B2 might inhibit UVA penetration, thus reducing antibacterial effect.This experimental exposure-model for possible treatment of keratitis, could be used to examine the possible eradication of other bacteria using UVA and B2.
Keywords: bacterial disease • keratitis • keratoconus