April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Treatment of Keratitis?, A Model for the Possibility to Evaluate Bacterial Eradication Using UVA+ Riboflavin
Author Affiliations & Notes
  • A. H. Backman
    Clinical Research Center, Clinical Reserch Centre, Orebro, Sweden
    Department of Ophtamology, University Hospital, Orebro, Sweden
  • K. Makdoumi
    Department of Ophtamology, University Hospital, Orebro, Sweden
  • J. Mortensen
    Department of Ophtamology, University Hospital, Orebro, Sweden
  • S. Crafoord
    Department of Ophtamology, University Hospital, Orebro, Sweden
  • Footnotes
    Commercial Relationships  A.H. Backman, None; K. Makdoumi, None; J. Mortensen, None; S. Crafoord, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 3888. doi:
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      A. H. Backman, K. Makdoumi, J. Mortensen, S. Crafoord; Treatment of Keratitis?, A Model for the Possibility to Evaluate Bacterial Eradication Using UVA+ Riboflavin. Invest. Ophthalmol. Vis. Sci. 2010;51(13):3888.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To evaluate bacterial eradication using UVA + Riboflavin used in Collagen Cross-Linking.

Methods: : S. epidermidis

Results: : Almost complete eradication( approx. 83 %) was achived using B2 (100 to 500 uM) and UVA for 60 minutes(10.8 J/m2). At higher concentrations of B2,1000 µM, 2000 µM and 2430 µM, the eradication was only around 40-60%. The thickness of the reaction-fluid could influence the UVA-exposure time for eradication as well as high B2-concentrations.A thinner layer was difficult to maintain as a complete surface over the entire area for UVA-exposure. Controls were significantly different. Increased UVA-dose was more efficient then higher B2-konc.

Conclusions: : The UVA/B2 combination used in Collagen Cross-Linking can eradicate bacteria in fluid solutions. The combined effect was more efficient than UVA alone. The variations of B2- concentration seems to be of importance for the degree of bacterial reduction and a rather low dose seemed to be more efficient than a higher one. Excess B2 might inhibit UVA penetration, thus reducing antibacterial effect.This experimental exposure-model for possible treatment of keratitis, could be used to examine the possible eradication of other bacteria using UVA and B2.

Keywords: bacterial disease • keratitis • keratoconus 
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