April 2010
Volume 51, Issue 13
ARVO Annual Meeting Abstract  |   April 2010
Treatment of Fungal Keratitis Infection by Fusarium With Corneal Crosslinking
Author Affiliations & Notes
  • M. Berra
    Laboratorio de Investigaciones Oculares, Buenos Aires, Argentina
  • G. Galperin
    Laboratorio de Investigaciones Oculares, Buenos Aires, Argentina
  • I. Berra
    BioFundus, Buenos Aires, Argentina
  • G. Boscaro
    BioFundus, Buenos Aires, Argentina
  • A. Berra
    Laboratorio de Investigaciones Oculares, Buenos Aires, Argentina
  • Footnotes
    Commercial Relationships  M. Berra, None; G. Galperin, None; I. Berra, None; G. Boscaro, None; A. Berra, None.
  • Footnotes
    Support  UBACYT 403 and PICT 2007-2252
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 3908. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      M. Berra, G. Galperin, I. Berra, G. Boscaro, A. Berra; Treatment of Fungal Keratitis Infection by Fusarium With Corneal Crosslinking. Invest. Ophthalmol. Vis. Sci. 2010;51(13):3908.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Purpose: : To evaluate the efficacy of Corneal Crosslinking (Riboflavin UV-A). as a single therapy in Fusarium fungal keratitis

Methods: : 12 rabbits were systemic anesthetized and theirs right corneal was inoculated into the stroma in five areas (central, up, bottom, right, left) with 20 ul each inoculation with Fusarium sp (10 (5) UFC/ml), furthermore, right corneas were scratched eight times in a criss-cross pattern with a 30 gauge needle and fifty ul of Fusarium sp (10 (5) UFC/ml), were instilled in the cul-de-sac.Rabbits were divided in two groups: group 1 (G1) with treatment, group 2 (G2) used as a control group.72 hs after infection, G1 were treated with crosslinking associated with Riboflavine. Complete desepithelialization was performed manually in treated corneas, Dextran 0.1% Riboflavin 20% was drop every 5 minutes for 30 minutes and then after checking the equipment's energy (UV 370 nm. Energy 3mW / cm2) irradiation of the stromal bed was started. During this period of time, riboflavine was applied every 5 minutes. Animals of G2 did not received any treatment.96 hours later, the right eyes were evaluated with biomicroscopy, enuclated and sent:1) 6 corneal buttons sent to microbiology, (G1:n=3 and G2:n=3), where they were weighed, corneal rupture was performed in sterile mortar (one for each cornea), cultured in Sabouraud medium agar plate, incubation at 28 ° C for 7 days, count colonies on the six plates.2) 6 corneal buttons sent for histological examination, (G1=3 and G2=3), where hematoxylin-eosin, Pas and Giemsa stainings were performed.

Results: : Clinical Evaluation: None of the treated corneas presented slit-lamp appearance of greater infiltration than the ontrol ones at the time of enucleation.Microbiology laboratory: G1: 18.9±15.1 colonies per gr of cornea (col/gr co) and G2 41.8 ±11.2 (col/gr co) (p<0.05)Pathological study: G1: 2/3 corneas showed Fusarium hiphas and inflammatory cells and G3: 3/3 corneas showed Fusarium hiphas and inflammatory cells. Numbers of hyphas and inflammatory cells were higher in G1 than G2

Conclusions: : Treatment of fungal keratitis with Corneal Crosslinking (Riboflavin UV-A) appears to be effective in decreasing the intensity and severity of infectious keratitis by Fusarium.We concluded that this therapy may be considered useful as a coadyuvant to the medical treatment or as a resource in resistent infections.

Keywords: cornea: stroma and keratocytes • fungal disease • keratitis 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.