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Y. Wang, H. Zhang, C. Jia, S. Li, H. Xi, S. Ren, F. Zhang, W. Song; Involvement of Complement Factor B in Pathogenesis of Fungal Keratitis. Invest. Ophthalmol. Vis. Sci. 2010;51(13):3909.
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In preliminary study, we found that humoral immunity plays animportant role in the pathogenesis and prognosis of experimentalfungal keratitis (FK). This study further explores whether complementfactor B is involved in such a case.
Experimental murine FK was induced by intrastromal injectionof Candida albicans spores or Aspergillus fumigatus spores.Wild-type C57 mice and factor B gene knockout C57 (FBKO) micewere sued. Corneal diseases were scored on a 12-point scaleconsidering the area and depth of the lesion and the irregularityof corneal surface. Histopathology was utilized to detect inflammatorycells recruitment and fungal hyphae and spores infiltrationin diseased corneas.
Compared to wild-type C57 mice, FBKO mice manifested severercorneal disease and had a longer course of disease (P <0.05)when challenged with either Candida albicans (Fig1) or Aspergillusfungal keratitis. Histological studies showed that corneas frominfected FBKO mice contained more hyphae than those from controlones (Fig2). Also, cellular infiltration in infected FBKO corneaswere prolonged than in control.
FBKO mice were more vulnerable to fungal infection and showeda longer disease course than wild-type C57 mice, suggestingthat factor B play an important role in the pathogenesis andprognosis of FK.
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