April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Involvement of Complement Factor B in Pathogenesis of Fungal Keratitis
Author Affiliations & Notes
  • Y. Wang
    Provincial Key Lab of Ophthalmology, Shandong Eye Institute, Qingdao, China
  • H. Zhang
    Provincial Key Lab of Ophthalmology, Shandong Eye Institute, Qingdao, China
  • C. Jia
    Provincial Key Lab of Ophthalmology, Shandong Eye Institute, Qingdao, China
  • S. Li
    Provincial Key Lab of Ophthalmology, Shandong Eye Institute, Qingdao, China
  • H. Xi
    Provincial Key Lab of Ophthalmology, Shandong Eye Institute, Qingdao, China
  • S. Ren
    Provincial Key Lab of Ophthalmology, Shandong Eye Institute, Qingdao, China
  • F. Zhang
    Provincial Key Lab of Ophthalmology, Shandong Eye Institute, Qingdao, China
  • W. Song
    Institute for Translational Medicine and Therapeutics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania
  • Footnotes
    Commercial Relationships  Y. Wang, None; H. Zhang, None; C. Jia, None; S. Li, None; H. Xi, None; S. Ren, None; F. Zhang, None; W. Song, None.
  • Footnotes
    Support  National Science Foundation of China 30630063 and 973 Project 2009CB526506
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 3909. doi:
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    • Get Citation

      Y. Wang, H. Zhang, C. Jia, S. Li, H. Xi, S. Ren, F. Zhang, W. Song; Involvement of Complement Factor B in Pathogenesis of Fungal Keratitis. Invest. Ophthalmol. Vis. Sci. 2010;51(13):3909.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose:
 

In preliminary study, we found that humoral immunity plays animportant role in the pathogenesis and prognosis of experimentalfungal keratitis (FK). This study further explores whether complementfactor B is involved in such a case.

 
Methods:
 

Experimental murine FK was induced by intrastromal injectionof Candida albicans spores or Aspergillus fumigatus spores.Wild-type C57 mice and factor B gene knockout C57 (FBKO) micewere sued. Corneal diseases were scored on a 12-point scaleconsidering the area and depth of the lesion and the irregularityof corneal surface. Histopathology was utilized to detect inflammatorycells recruitment and fungal hyphae and spores infiltrationin diseased corneas.

 
Results:
 

Compared to wild-type C57 mice, FBKO mice manifested severercorneal disease and had a longer course of disease (P <0.05)when challenged with either Candida albicans (Fig1) or Aspergillusfungal keratitis. Histological studies showed that corneas frominfected FBKO mice contained more hyphae than those from controlones (Fig2). Also, cellular infiltration in infected FBKO corneaswere prolonged than in control.

 
Conclusions:
 

FBKO mice were more vulnerable to fungal infection and showeda longer disease course than wild-type C57 mice, suggestingthat factor B play an important role in the pathogenesis andprognosis of FK.  

 

 
Keywords: keratitis • fungal disease • immunomodulation/immunoregulation 
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