April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Altered Ganglioside Expression Modulates the Pathogenic Mechanism of Thyroid Associated Ophthalmopathy by Increase in Hyaluronan
K. Kook; Y.-H. Choi; Y. Kim; S. Park; I. Jou; S. Kim; S. Lee
Author Affiliations & Notes
  • K. Kook
    Department of Ophthalmology, Ajou University School of Medicine, Suwon, Republic of Korea
  • Y.-H. Choi
    Department of Physiology, Ewha Womans University College of Medicine, Seoul, Republic of Korea
  • Y. Kim
    Ajou University School of Medicine, Chronic Inflammatory Disease Rearch Center, Suwon, Republic of Korea
  • S. Park
    Ajou University School of Medicine, Chronic Inflammatory Disease Rearch Center, Suwon, Republic of Korea
  • I. Jou
    Ajou University School of Medicine, Chronic Inflammatory Disease Rearch Center, Suwon, Republic of Korea
  • S. Kim
    Department of Ophthalmology, Kim's Eye Hospital, Seoul, Republic of Korea
  • S. Lee
    Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships  K. Kook, None; Y.-H. Choi, None; Y. Kim, None; S. Park, None; I. Jou, None; S. Kim, None; S. Lee, None.
  • Footnotes
    Support  KOSEF Grant R13-2003-019-01004-0 and KRF Grant KRF-2008-331-E00023
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 3926. doi:
  • Views
  • Share
  • Tools
    • Alerts
      User Alerts
      You are adding an alert for:
      Altered Ganglioside Expression Modulates the Pathogenic Mechanism of Thyroid Associated Ophthalmopathy by Increase in Hyaluronan
      You will receive an email whenever this article is corrected, updated, or cited in the literature. You can manage this and all other alerts in My Account
      The alert will be sent to:
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation
      Citation

      K. Kook, Y.-H. Choi, Y. Kim, S. Park, I. Jou, S. Kim, S. Lee; Altered Ganglioside Expression Modulates the Pathogenic Mechanism of Thyroid Associated Ophthalmopathy by Increase in Hyaluronan. Invest. Ophthalmol. Vis. Sci. 2010;51(13):3926.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

      ×
    • Get Permissions
  • Supplements
Abstract

Purpose: : To reveal the role of gangliosides in pathogenic mechanisms of Thyroid associated ophthalmopathy (TAO)

Methods: : We investigated the ganglioside profile in the orbital tissue of patients with TAO and examined the effect of exogenous gangliosides on the expression of hyaluronan and the morphology of orbital fibroblasts from subjects without inflammatory disease.

Results: : Trisialoganglioside 1b (GT1b) was significantly overexpressed in the orbital tissue of TAO patients compared to control tissue, while no significant difference was observed for either monosialoganglioside 1 (GM1) or disialoganglioside 1a (GD1a). Moreover, mRNA levels of sialyltransferase I (SAT I) and SAT II were increased in TAO patients compared to control. GT1b-treated cells contained sparse flocculent precipitates in lysosomes due, at least in part, to an increase in hyaluronan. Correspondingly, the level of extracellular hyaluronan measured was increased in GT1b-treated orbital fibroblasts. The morphologic changes of orbital fibroblasts were induced by GT1b, but not by GM1 or GD1a. And these changes in GT1b-treated orbital fibroblasts were nicely attenuated by co-treatment with hyaluronidase.

Conclusions: : Our results suggest that gangliosides, particularly GT1b, may play a role in the pathologic mechanisms of TAO by stimulating an increase in hyaluronan.

Keywords: orbit • autoimmune disease 
© 2010, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2015 Association for Research in Vision and Ophthalmology.
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×
This site uses cookies. By continuing to use our website, you are agreeing to our privacy policy.Accept