April 2010
Volume 51, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2010
Comparative in vitro Secretion of TGF-β1 and Proteinases by wt and rd1 Mouse Retinal Explants
Author Affiliations & Notes
  • S. Ahuja
    Ophthalmology, Clinical Sciences, Lund,
    Lund University, Lund, Sweden
  • P. Ahuja-Jensen
    Ophthalmology, Clinical Sciences, Lund,
    Lund University, Lund, Sweden
  • A. Caffe
    Ophthalmology, Clinical Sciences, Lund,
    Lund University, Lund, Sweden
  • M. Abrahamson
    Clinical Chemistry and Pharmacology, Lund University Hospital, Lund, Sweden
  • P. A. Ekstrom
    Ophthalmology, Clinical Sci Lund,
    Lund University, Lund, Sweden
  • T. van Veen
    Ophthalmology, Clinical Sciences, Lund,
    Lund University, Lund, Sweden
  • Footnotes
    Commercial Relationships  S. Ahuja, None; P. Ahuja-Jensen, None; A. Caffe, None; M. Abrahamson, None; P.A. Ekstrom, None; T. van Veen, None.
  • Footnotes
    Support  Dutch Retina Foundation, KMA (Sweden), Stiftelsen Synfrämjandets Forskningsfond (Sweden); Stiftelsen för Synskadade i f d Malmöhus Län (Sweden), FFB, VRM
Investigative Ophthalmology & Visual Science April 2010, Vol.51, 4040. doi:
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    • Get Citation

      S. Ahuja, P. Ahuja-Jensen, A. Caffe, M. Abrahamson, P. A. Ekstrom, T. van Veen; Comparative in vitro Secretion of TGF-β1 and Proteinases by wt and rd1 Mouse Retinal Explants. Invest. Ophthalmol. Vis. Sci. 2010;51(13):4040.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Due to Pde6brd1 gene mutation in β subunit of phosphodiesterase, rd1 mouse retina shows degeneration homologous to a form of Retinitis Pigmentosa. The secretion of TGF-β1, matrix metalloproteinases (MMPs) and cathepsins and their endogenous inhibitors TIMPs and cystatin C by wt and retinal degeneration (rd1) mouse retinas was compared to understand the mechanism of retinal degeneration.

Methods: : Retinal explants from postnatal day 2 (PN2) and PN7 wt and rd1 mice were cultured and the retinal conditioned medium (RCM) was collected at PN12 and PN21 and analyzed. TGF- β1, MMPs / TIMPs, and cathepsins / cystatin C secreted into the RCM were determined by ELISA.

Results: : In vitro

Conclusions: : In wt explants higher in vitro levels of TGF-β1 may transiently increase the secretion of MMPs and cathepsins which activate TGF-β1 and remodel the ECM. In rd1 retina consistent increases in the expression of proteinases relative to their inhibitors could degrade TGF-β1, ECM and possibly lead to retinal degeneration. Therapeutic treatment with individual proteinase inhibitors and a combination(s) of inhibitor(s) needs investigation.

Keywords: proteolysis • retinal degenerations: cell biology • growth factors/growth factor receptors 
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