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Y. Ji, E.-J. Lee, N. Grzywacz; Ring-Like Organization of Cones in S334ter-Line-3 Transgenic Rats. Invest. Ophthalmol. Vis. Sci. 2010;51(13):4078.
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© ARVO (1962-2015); The Authors (2016-present)
Previous work has described changes in the morphology, location, and/or expression of horizontal, bipolar, and amacrine cells due to retinal degeneration. However, little is known about the degeneration effects on cone photoreceptor cells. We investigated the remodeling of cones (morphology, orientation, and organization) in developing S334ter-line-3 rat retinas.
S334ter-line-3 rats were used as a model of retinal degeneration. Control animals were age-matched Sprague Dawley rats. Sacrifice of rats took place at post-natal (P) days P30, P90, and P180. Beforehand, control and RD rats were on a daily 12 h light/dark cycle. We studied the morphology, orientation, and distribution of s-opsin- and m-opsin-positive cells in all rats by immunohistochemistry.
The number of cones showed no significant difference between P180-control and P180 RD retinas. However, in P30-RD retinas, the outer-nuclear layers were significantly thinner compared to control retinas. Moreover, RD cones showed shortening of their segments and axons. From P60 onward, cones appeared at the outer margin of the inner nuclear layer of RD retinas. In the P30-, P60-, and P180-control whole-mount retinas, the spatial organization of cones showed random, salt-and-pepper distribution. In contrast, the spatial distribution of cones in S334ter-line-3 whole-mount retinas exhibited multiple ring-like shapes. In those cases, the cones’ axons pointed towards the center of the rings, whereas their dendrites were in the opposite direction. Double-labeling experiments showed no co-localization between S-opsin and M-opsin immunoreactive cells, but their rings were in the same positions in space.
The RD rat’s retina undergoes extensive changes in the morphology, orientation, and spatial distribution of cones. The spatial reorganization into ring-like shapes is not due to cell death at their centers but to migration. This is because RD and control retinas have the same number of cones.This work was funded by National Eye Institute Grant EY11170 to NMG, and by a Fight for Sight and a James H. Zumberge Research Grants to E-JL.
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